論文

査読有り
2016年10月

A host cell membrane microdomain is a critical factor for organelle discharge by Toxoplasma gondii

PARASITOLOGY INTERNATIONAL
  • Michiru Tahara
  • ,
  • Syed Bilal Ahmad Andrabi
  • ,
  • Ryuma Matsubara
  • ,
  • Hiroka Aonuma
  • ,
  • Kisaburo Nagamune

65
5
開始ページ
378
終了ページ
388
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.parint.2016.05.012
出版者・発行元
ELSEVIER IRELAND LTD

Host cell microdomains are involved in the attachment, entry, and replication of intracellular microbial pathogens. Entry into the host cell of Toxoplasma gondii and the subsequent survival of this protozoan parasite are tightly coupled with the proteins secreted from organelle called rhoptry. The rhoptry proteins are rapidly discharged into clusters of vesicles, called evacuoles, which are then delivered to parasitophorous vacuoles (PVs) or nucleus. In this study, we examined the roles of two host cell microdomain components, cholesterol and glycosylphosphatidylinositol (GPI), in evacuole formation. The acute depletion of cholesterol from the host cell plasma membrane blocked evacuole formation but not invasion. Whereas the lack of host cell GPI also altered evacuole formation but not invasion, instead inducing excess evacuole formation. The latter effect was not influenced by the evacuole-inhibiting effects of host cell cholesterol depletion, indicating the independent roles of host GPI and cholesterol in evacuole formation. In addition, the excess formation of evacuoles resulted in the enhanced recruitment of host mitochondria and endoplasmic reticulum to PVs, which in turn stimulated the growth of the parasite. (C) 2016 The Authors. Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NC-ND license.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1016/j.parint.2016.05.012
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27217289
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000381593200004&DestApp=WOS_CPL

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