2020年9月1日
Multi-photon fluorescence microscopy imaging of mitochondria in living cells excited by Yb-doped femtosecond fiber laser utilizing two- And three-photon competitive absorption
AIP Advances
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- 巻
- 10
- 号
- 9
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1063/5.0004953
© 2020 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license By employing an Yb-doped femtosecond fiber laser oscillating at 1030 nm, multi-photon fluorescence microscopy imaging was demonstrated, utilizing competitive two- and three-photon absorption processes for excitation of the fluorescence probes. Competitive two- and three-photon absorptions occurred for all six of the fluorescence probes employed. The mitochondria of human embryonic kidney 293 cells were stained with the probes, and multi-photon fluorescence images were obtained in all cases. The brightest multi-photon fluorescence image was obtained when employing the probe compound possessing the longest cut-off wavelength. This can be attributed to the larger contribution of two-photon absorption to the competitive excitation process. Thus, by utilizing competitive two- and three-photon absorption processes, multi-photon fluorescence images can be acquired using Yb-doped femtosecond fiber laser excitation with a variety of probes.
- リンク情報
- ID情報
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- DOI : 10.1063/5.0004953
- eISSN : 2158-3226
- SCOPUS ID : 85092671471