論文

査読有り
2017年4月

Single-molecule detection of protein efflux from microorganisms using fluorescent single-walled carbon nanotube sensor arrays

NATURE NANOTECHNOLOGY
  • Markita Patricia Landry
  • ,
  • Hiroki Ando
  • ,
  • Allen Y. Chen
  • ,
  • Jicong Cao
  • ,
  • Vishal Isaac Kottadiel
  • ,
  • Linda Chio
  • ,
  • Darwin Yang
  • ,
  • Juyao Dong
  • ,
  • Timothy K. Lu
  • ,
  • Michael S. Strano

12
4
開始ページ
368
終了ページ
377
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/NNANO.2016.284
出版者・発行元
NATURE PUBLISHING GROUP

A distinct advantage of nanosensor arrays is their ability to achieve ultralow detection limits in solution by proximity placement to an analyte. Here, we demonstrate label-free detection of individual proteins from Escherichia coli (bacteria) and Pichia pastoris (yeast) immobilized in a microfluidic chamber, measuring protein efflux from single organisms in real time. The array is fabricated using non-covalent conjugation of an aptamer-anchor polynucleotide sequence to near-infrared emissive single-walled carbon nanotubes, using a variable chemical spacer shown to optimize sensor response. Unlabelled RAP1 GTPase and HIV integrase proteins were selectively detected from various cell lines, via large near-infrared fluorescent turn-on responses. We show that the process of E. coli induction, protein synthesis and protein export is highly stochastic, yielding variability in protein secretion, with E. coli cells undergoing division under starved conditions producing 66% fewer secreted protein products than their non-dividing counterparts. We further demonstrate the detection of a unique protein product resulting from T7 bacteriophage infection of E. coli, illustrating that nanosensor arrays can enable real-time, single-cell analysis of a broad range of protein products from various cell types.

Web of Science ® 被引用回数 : 81

リンク情報
DOI
https://doi.org/10.1038/NNANO.2016.284
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28114298
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000398767500018&DestApp=WOS_CPL

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