論文

査読有り
2018年7月1日

Recovery of mycobacteriophages from archival stocks stored for approximately 50 years in Japan

Archives of Virology
  • Takako Ujihara
  • ,
  • Jumpei Uchiyama
  • ,
  • Tadahiro Nasukawa
  • ,
  • Hiroki Ando
  • ,
  • Hironobu Murakami
  • ,
  • Naoya Ohara
  • ,
  • Midori Ogawa
  • ,
  • Toshio Yamazaki
  • ,
  • Masanori Daibata
  • ,
  • Masahiro Sakaguchi
  • ,
  • Shigenobu Matsuzaki

163
7
開始ページ
1915
終了ページ
1919
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00705-018-3788-8

© 2018, Springer-Verlag GmbH Austria, part of Springer Nature. Mycobacteriophage archival stocks have been kept for ca. 20–50 years in Japan. In this study, we attempted to recover mycobacteriophages from 50 archival stocks and briefly analyzed the recovered phages. The phages were recovered from 72.2% (13/18) of the lyophilized stocks that had been stored for 47-56 years. Moreover, the analysis of 12 representative recovered phages led to their classification as belonging to the family Siphoviridae, and seven of them were typed by polymerase chain reaction (PCR) targeting the gene that encodes the tape measure protein. Considering these results, lyophilization seems to be suitable for phage archival storage.

リンク情報
DOI
https://doi.org/10.1007/s00705-018-3788-8
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29511830
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85048470792&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85048470792&origin=inward

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