論文

査読有り 国際誌
2019年5月16日

Different effects of partial pressure in a high-pressure gaseous mixture of carbon monoxide and oxygen for rat heart preservation.

Scientific reports
  • Naoyuki Hatayama
  • ,
  • Shuichi Hirai
  • ,
  • Kaori Fukushige
  • ,
  • Hiroki Yokota
  • ,
  • Masahiro Itoh
  • ,
  • Munekazu Naito

9
1
開始ページ
7480
終了ページ
7480
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-019-43905-0

We maintained the function of an extracted rat heart after 24-48 h preservation in a high-pressure gaseous mixture of carbon monoxide (CO) and oxygen (O2). Here, we assessed the effects of different partial pressures of hyperbaric CO and O2 for 24-48 h at 4 °C on rat heart preservation and compared conditions including immersion in University of Wisconsin solution. Preserved hearts were transplanted into recipient rats via heterotopic cervical heart transplantation for in vivo evaluation and perfused using the Langendorff system for ex vivo evaluation. The survival rate of transplanted hearts was 100% at postoperative day 7 in the CO + O2 (PCO:PO2 = 1.5:2.0 atm) group but only 33% in the CO + O2 (PCO:PO2 = 2.0:1.5 atm) group. Langendorff system and histopathological analysis revealed that the left ventricular pressure of preserved hearts in the CO + O2 (PCO:PO2 = 1.5:2.0 atm) group was better than the CO + O2 (PCO:PO2 = 2.0:1.5 atm). We demonstrate that exposure of rat hearts to hyperbaric CO and O2 is superior to the immersion method and that partial pressure of hyperbaric CO and O2 is crucial to preservation.

リンク情報
DOI
https://doi.org/10.1038/s41598-019-43905-0
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31097781
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6522590
ID情報
  • DOI : 10.1038/s41598-019-43905-0
  • PubMed ID : 31097781
  • PubMed Central 記事ID : PMC6522590

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