論文

査読有り 国際誌
2020年5月

Sequentially addressable dielectrophoretic array for high-throughput sorting of large-volume biological compartments.

Science advances
  • A Isozaki
  • Y Nakagawa
  • M H Loo
  • Y Shibata
  • N Tanaka
  • D L Setyaningrum
  • J-W Park
  • Y Shirasaki
  • H Mikami
  • D Huang
  • H Tsoi
  • C T Riche
  • T Ota
  • H Miwa
  • Y Kanda
  • T Ito
  • K Yamada
  • O Iwata
  • K Suzuki
  • S Ohnuki
  • Y Ohya
  • Y Kato
  • T Hasunuma
  • S Matsusaka
  • M Yamagishi
  • M Yazawa
  • S Uemura
  • K Nagasawa
  • H Watarai
  • D Di Carlo
  • K Goda
  • 全て表示

6
22
開始ページ
eaba6712
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1126/sciadv.aba6712

Droplet microfluidics has become a powerful tool in precision medicine, green biotechnology, and cell therapy for single-cell analysis and selection by virtue of its ability to effectively confine cells. However, there remains a fundamental trade-off between droplet volume and sorting throughput, limiting the advantages of droplet microfluidics to small droplets (<10 pl) that are incompatible with long-term maintenance and growth of most cells. We present a sequentially addressable dielectrophoretic array (SADA) sorter to overcome this problem. The SADA sorter uses an on-chip array of electrodes activated and deactivated in a sequence synchronized to the speed and position of a passing target droplet to deliver an accumulated dielectrophoretic force and gently pull it in the direction of sorting in a high-speed flow. We use it to demonstrate large-droplet sorting with ~20-fold higher throughputs than conventional techniques and apply it to long-term single-cell analysis of Saccharomyces cerevisiae based on their growth rate.

リンク情報
DOI
https://doi.org/10.1126/sciadv.aba6712
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32524002
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7259936
ID情報
  • DOI : 10.1126/sciadv.aba6712
  • PubMed ID : 32524002
  • PubMed Central 記事ID : PMC7259936

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