論文

査読有り
2006年1月

SWI/SNF complex is essential for NRSF-mediated suppression of neuronal genes in human nonsmall cell lung carcinoma cell lines

ONCOGENE
  • H Watanabe
  • ,
  • T Mizutani
  • ,
  • T Haraguchi
  • ,
  • N Yamamichi
  • ,
  • S Minoguchi
  • ,
  • M Yamamichi-Nishina
  • ,
  • N Mori
  • ,
  • T Kameda
  • ,
  • T Sugiyama
  • ,
  • H Iba

25
3
開始ページ
470
終了ページ
479
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/sj.onc.1209068
出版者・発行元
NATURE PUBLISHING GROUP

Mammalian chromatin remodelingfact or, SWI/ SNF complex contains a single molecule of either Brm or BRG1 as the ATPase catalytic subunit. Here, we show that the SWI/ SNF complex forms a larger complex with neuron-restrictive silencer factor (NRSF) and its corepressors, mSin3A and CoREST, in human nonsmall cell lung carcinoma cell lines. We also demonstrate that the strong transcriptional suppression of such neuron-specific genes as synaptophysin and SCG10 by NRSF in these non-neural cells requires the functional SWI/ SNF complex; these neuronal genes were elevated in cell lines deficient in both Brm and BRG1, whereas retrovirus vectors expressing siRNAs targeting integral components of SWI/ SNF complex (Brm/BRG1 or Ini1) induced expression of these neuronal genes in SWI/ SNF-competent cell lines. In cell lines deficient in both Brm and BRG1, exogenous Brm or BRG1 suppressed expression of these neuronal genes in an ATP-dependent manner and induced efficient and specific deacetylation of histone H4 around the NRSF binding site present in the synaptophysin gene by a large complex containing the recruited functional SWI/ SNF complex. Patients with Brm/BRG1 deficient lung carcinoma have been reported to carry poor prognosis; derepression of NRSF-regulated genes including these neuron-specific genes could contribute to enhance tumorigenicity and also would provide selective markers for Brm/BRG1-deficient tumors.

リンク情報
DOI
https://doi.org/10.1038/sj.onc.1209068
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/16247481
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000234714100015&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/sj.onc.1209068
  • ISSN : 0950-9232
  • PubMed ID : 16247481
  • Web of Science ID : WOS:000234714100015

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