1998年7月
Differential withdrawal of retinal axons induced by a secreted factor
JOURNAL OF NEUROSCIENCE
- ,
- 巻
- 18
- 号
- 13
- 開始ページ
- 5008
- 終了ページ
- 5018
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- SOC NEUROSCIENCE
To understand the development of the topographic map in the chick retinotectal projection, we studied the long-term interactions between retinal axons and tectal cell processes using a novel coculture system, the ryomen chamber. Both nasal and temporal retinal axons initially grew equally well on a substrate consisting of posterior tectal cell processes; however, subsequently most temporal axons withdrew from this surface, whereas most nasal axons did not. Experiments using conditioned media indicate that posterior tectal cells induced withdrawal of the temporal axons by secreting a soluble factor. This withdrawal seems to be distinct from the immediate repulsive effect of ephrin-A2 (ELF-1) and ephrin-A5 (RAGS) seen in the stripe assay because (1) the withdrawal-inducing factor was diffusible, whereas ephrin-A2 and -A5 are membrane-bound, and (2) the withdrawal-inducing factor appeared later in development than ephrin-A2 and -A5. Furthermore, sensitivity to the withdrawal-inducing factor decreased continuously from the temporal to nasal retina. These results suggest that target cell-induced axonal withdrawal may be involved during a late stage of the development of the retinotectal map.
- リンク情報
- ID情報
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- ISSN : 0270-6474
- CiNii Articles ID : 120001086985
- Web of Science ID : WOS:000074290000021