2015年11月5日
Quantitative nature of overexpression experiments.
Molecular biology of the cell
- 巻
- 26
- 号
- 22
- 開始ページ
- 3932
- 終了ページ
- 9
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1091/mbc.E15-07-0512
Overexpression experiments are sometimes considered as qualitative experiments designed to identify novel proteins and study their function. However, in order to draw conclusions regarding protein overexpression through association analyses using large-scale biological data sets, we need to recognize the quantitative nature of overexpression experiments. Here I discuss the quantitative features of two different types of overexpression experiment: absolute and relative. I also introduce the four primary mechanisms involved in growth defects caused by protein overexpression: resource overload, stoichiometric imbalance, promiscuous interactions, and pathway modulation associated with the degree of overexpression.
- リンク情報
- ID情報
-
- DOI : 10.1091/mbc.E15-07-0512
- ISSN : 1059-1524
- ORCIDのPut Code : 48124736
- PubMed ID : 26543202
- PubMed Central 記事ID : PMC4710226