論文

査読有り 国際誌
2011年

The expression of GM-CSF and osteopontin in immunocompetent cells precedes the odontoblast differentiation following allogenic tooth transplantation in mice

Journal of Histochemistry and Cytochemistry
  • Saito, K.
  • ,
  • Nakatomi, M.
  • ,
  • Ida-Yonemochi, H.
  • ,
  • Kenmotsu, S.
  • ,
  • Ohshima, H.

59
5
開始ページ
518
終了ページ
29
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1369/0022155411403314
出版者・発行元
SAGE PUBLICATIONS LTD

Dental pulp elaborates both bone and dentin under pathological conditions such as tooth replantation/transplantation. This study aims to clarify the expression of granulocyte macrophage colony-stimulating factor (GM-CSF) and osteopontin (OPN) in the process of reparative dentin formation by allogenic tooth transplantation using in situ hybridization for OPN and immunohistochemistry for GM-CSF and OPN at both levels of light and electron microscopes. Following the extraction of the mouse molar, the roots and pulp floor were resected and immediately allografted into the sublingual region. On days 1 to 3, immunocompetent cells such as macrophages and dendritic cells expressed both GM-CSF and OPN, and some of them were arranged along the pulp-dentin border and extended their cellular processes into the dentinal tubules. On days 5 to 7, tubular dentin formation commenced next to the preexisting dentin at the pulp horn where nestin-positive odontoblast-like cells were arranged. Until day 14, bone-like tissue formation occurred in the pulp chamber, where OPN-positive osteoblasts surrounded the bone matrix. These results suggest that the secretion of GM-CSF and OPN by immunocompetent cells such as macrophages and dendritic cells plays a role in the maturation of dendritic cells and the differentiation of odontoblasts, respectively, in the regenerated pulp tissue following tooth transplantation.

リンク情報
DOI
https://doi.org/10.1369/0022155411403314
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21430263
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3201177
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000293703400005&DestApp=WOS_CPL
Scopus Url
http://www.scopus.com/inward/record.url?eid=2-s2.0-79958812863&partnerID=MN8TOARS
ID情報
  • DOI : 10.1369/0022155411403314
  • ISSN : 0022-1554
  • ORCIDのPut Code : 64573463
  • PubMed ID : 21430263
  • PubMed Central 記事ID : PMC3201177
  • SCOPUS ID : 79958812863
  • Web of Science ID : WOS:000293703400005

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