論文

査読有り 国際誌
2017年5月

Fatty acid-binding protein 5 function in hepatocellular carcinoma through induction of epithelial-mesenchymal transition.

Cancer medicine
  • Takanori Ohata
  • Hideki Yokoo
  • Toshiya Kamiyama
  • Moto Fukai
  • Takeshi Aiyama
  • Yutaka Hatanaka
  • Kanako Hatanaka
  • Kenji Wakayama
  • Tatsuya Orimo
  • Tatsuhiko Kakisaka
  • Nozomi Kobayashi
  • Yoshihiro Matsuno
  • Akinobu Taketomi
  • 全て表示

6
5
開始ページ
1049
終了ページ
1061
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/cam4.1020
出版者・発行元
WILEY

Hepatocellular carcinoma (HCC) is a highly prevalent cancer with poor prognosis. The correlation between overexpression of fatty acid-binding protein 5 (FABP5) and malignant potential of tumor growth and metastasis in several cancers has been previously reported. However, the correlation between FABP5 expression and HCC malignant behavior remains unknown. We compared FABP5 expression and patient characteristics in paired HCC and adjacent noncancerous liver tissues from 243 patients who underwent surgical resection of primary HCC. Cell proliferation, invasion, and migration assays were performed in HCC cell lines overexpressing FABP5 or downregulated for FABP5. Tumor growths were monitored in xenograft model, and liver and lung metastasis models were established. In the 243 HCC patients, FABP5-positive staining (n = 139/243, 57.2%) was associated with poor prognosis and recurrence (P < 0.0001) and showed positive correlation with distant metastasis, tumor size and vascular invasion (P < 0.05). Cell proliferation, invasion, and migration in vitro were enhanced by upregulation of FABP5 and decreased by downregulation of FABP5 in HCC cell lines. Similar results in tumor formation and metastasis were obtained through in vivo analyses. PCR array results revealed upregulation of SNAI1 in FABP5-overexpressing HepG2 cells. Western blot analysis showed significantly increased expression of E-cadherin and ZO-1 and decreased SNAI1 expression and nuclear translocation of β-catenin by knockdown of FABP5. We revealed a significant role for FABP5 in HCC progression and metastasis through the induction of epithelial-to-mesenchymal transition. FABP5 may be a potential novel prognostic biomarker and new therapeutic target for HCC.

リンク情報
DOI
https://doi.org/10.1002/cam4.1020
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28374947
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5430096
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000401330300017&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/cam4.1020
  • ISSN : 2045-7634
  • PubMed ID : 28374947
  • PubMed Central 記事ID : PMC5430096
  • Web of Science ID : WOS:000401330300017

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