論文

査読有り 国際誌
2019年12月

Efficacy of gelatin hydrogels incorporating triamcinolone acetonide for prevention of fibrosis in a mouse model.

Regenerative therapy
  • Nao Nakajima
  • Satoru Hashimoto
  • Hiroki Sato
  • Kazuya Takahashi
  • Takuro Nagoya
  • Kenya Kamimura
  • Atsunori Tsuchiya
  • Junji Yokoyama
  • Yuichi Sato
  • Hanako Wakatsuki
  • Masayuki Miyata
  • Yusuke Akashi
  • Ryusuke Tanaka
  • Ken Matsuda
  • Yasuhiko Tabata
  • Shuji Terai
  • 全て表示

11
開始ページ
41
終了ページ
46
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.reth.2019.04.001

Introduction: Triamcinolone acetonide (TA), a steroid, is often used clinically to prevent dysfunctions associated with fibrosis. The objective of this study was to examine whether TA can be suspended in a gelatin sheet for tissue engineering using a mouse skin wound model. Methods: TA was suspended in biodegradable gelatin and freeze-dried in a sheet form. The sheet was analyzed for homogeneity and controlled release of TA by high-performance liquid chromatography. We made two skin wounds on the dorsal side of mice. Gelatin sheets with TA (TA sheet) and without TA (control sheet) were attached to each skin wound. To determine the efficacy of the prepared TA sheet on the skin wounds, TA-sheet versus TA-injection experiments were conducted. Hematoxylin and eosin staining was performed to assess the grade of epithelialization and alpha smooth muscle actin (α-SMA) immunohistochemical staining was conducted to evaluate myofibroblast infiltration. Results: In the TA-release test in vitro, 7.7 ± 2.3% of TA was released from the sheet by 24 h. After replacing the initial phosphate-buffered saline (PBS) with collagenase PBS, the amount of released TA increased over time. The wound area/original skin wound area after 15 days with the TA sheet was significantly larger than that with the control sheet (26.9 ± 5.5% vs 10.7 ± 2.6%, p = 0.023). The α-SMA positive area/whole area with the TA sheet was significantly lower than that with the control sheet (4.65 ± 0.66% vs 7.24 ± 0.7%, p = 0.023). Furthermore, the α-SMA positive area/whole area with the TA sheet was significantly lower than that with TA injection (5.32 ± 0.45% vs 7.93 ± 0.75%, p = 0.013). Conclusions: We developed a TA sheet and confirmed both the homogeneity of the suspended TA and controlled-release of the TA in the presence of collagenase in vitro. The TA sheet caused less myofibroblast infiltration into the tissue than the control sheet or TA injection did.

リンク情報
DOI
https://doi.org/10.1016/j.reth.2019.04.001
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31193122
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6518320
ID情報
  • DOI : 10.1016/j.reth.2019.04.001
  • PubMed ID : 31193122
  • PubMed Central 記事ID : PMC6518320

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