Hiroyuki Kawahara

J-GLOBAL         Last updated: Dec 3, 2019 at 14:49
Hiroyuki Kawahara
Tokyo Metropolitan University

Research Interests


Research Areas


Academic & Professional Experience

Apr 2020
Professor, Faculty of Science, Tokyo Metropolitan University
Apr 2017
Director of the International Student Division, International Center, Tokyo Metropolitan University

Published Papers

A molecular triage process mediated by RING finger protein 126 and BAG6 regulates degradation of G0/G1 switch gene 2.
Kamikubo, K., Kato, H., Kioka, H., Yamazaki, S., Tsukamoto, O., Nishida, Y., Asano, Y., Imamura, H., Kawahara, H., Shintani, Y., and Takashima, S.
J. Biol. Chem.   294 14562-14573   Aug 2019   [Refereed]
Cytoplasmic control of Rab-family small GTPases through BAG6.
Takahashi, T., Minami, S., Tajima, K., Tsuchiya, Y., Sakai, N., Suga, K., Hisanaga, S., Obayashi, N., Fukuda, M., and Kawahara, H.
EMBO Rep.   e46794    Apr 2019   [Refereed]
TAS-203, an oral phosphodiesterase 4 inhibitor, suppresses goblet cell hyperplasia and MUC5AC production in rodent models.
Demizu, S., Asaka, M., Kawahara, H. and Sasaki, E.
Eur. J. Pharmacol.   849 22-29   Feb 2019   [Refereed]
Deubiquitinases USP5 and USP13 are recruited to and regulate heat-induced stress granules by deubiquitinating activities.
Xuan, X., Matsumoto, S., Endo, S., Fukushima, A., Kawahara, H., Saeki, Y., and Komada, M.
J. Cell Sci.   131 1-11   Mar 2018   [Refereed]
ZFP36L2 is a cell cycle-regulated CCCH-protein necessary for DNA lesion-induced S-phase arrest.
Noguchi, A., Adachi, S., Yokota, N., Hatta, T., Natsume, T., and Kawahara, H.
Biol. Open   7    Jan 2018   [Refereed]
Elimination of a signal-sequence uncleaved form of defective HLA protein through BAG6.
Yamamoto, K., Hayashishita, M., Minami, S., Suzuki, K., Hagiwara, T., Noguchi, A., and Kawahara, H.
Sci. Rep.   7    Oct 2017   [Refereed]
UBQLN4 recognizes mislocalized transmembrane domain proteins and targets these to proteasomal degradation.
Suzuki, R. and Kawahara, H.
EMBO Rep.   17 842-857   Apr 2016   [Refereed]
A conserved island of BAG6/Scythe is related to ubiquitin domains and participates in short hydrophobicity recognition.
Tanaka, H., Takahashi, T., Xie, Y., Minami, R., Yanagi, Y., Hayashishita, M., Suzuki, R., Yokota, N., Shimada, M., Mizushima, T., Kuwabara, N., Kato, R., and Kawahara, H.
FEBS J   283 662-677   Mar 2016   [Refereed]
Two degradation pathways of the p35 Cdk5 activation subunit, dependent and independent of ubiquitination.
Takasugi, T., Saito, T., Asada, A., Kawahara, H. and Hisanaga, S.
J. Biol. Chem.   291 4649-4657   Jan 2016   [Refereed]
Structure of a BAG6 (Bcl-2-associated athanogene 6)-Ubl4a (ubiquitin-like protein 4a) complex reveals a novel binding interface that functions in tail-anchored protein biogenesis.
Kuwabara, N., Minami, R., Yokota, N., Matsumoto, H., Senda, T., Kawahara, H. (Corresponding author), and Kato, R.
J. Biol. Chem.   290 9387-9398   Jan 2015   [Refereed]
BAG6/BAT3: Emerging roles in quality control for nascent polypeptides.
Kawahara, H., Minami, R. and Yokota, N.
J. Biochem. (JB Review)   153 147-160   Jan 2013   [Refereed][Invited]
Calpastatin, an endogenous calpain-inhibitor protein, regulates the cleavage of the Cdk5 activator p35 to p25.
Sato, K., Minegishi, S., Takano, J., Plattner, F., Saito, T., Asada, A., Kawahara, H., Iwata, N., Saido, T.C., and Hisanaga, S.
J. Neurochem.   117 504-511   Jan 2011   [Refereed]
Ojima, K., Y Kawabata, Y., Nakao, H., Nakao, K., Doi, N., Kitamura, F., Ono, Y., Hata, S., Suzuki, H., Kawahara, H., Labeit, S., Toyama-Sorimachi, N., Suzuki, K., Maeda, T., Abe, K., Aiba, A., and Sorimachi, H.
J. Clin. Invest.   120 2672-2683   Apr 2010   [Refereed]
Minami, R., Hayakawa, A., Kagawa, H., Yanagi, Y., Yokosawa, H., and Kawahara, H.
J. Cell Biol.   190(4) 637-650   Aug 2010   [Refereed]
Commitment of 1-methyl-4-phenylpyrinidinium ion-induced neuronal cell death by proteasome-mediated degradation of p35 Cyclin-dependent kinase 5 activator.
Endo, R., Saito, T., Asada, A., Kawahara, H. and Hisanaga, S.
J. Biol. Chem.   284 26029-26039   Jun 2009   [Refereed]
Rpn10-mediated degradation of ubiquitinated proteins is essential for mouse development.
Hamazaki, J., Sasaki, K., Kawahara, H., Hisanaga, S., Tanaka, K., and Murata, S.
Mol. Cell. Biol.   27 6629-6638   Jun 2007   [Refereed]
Minami, R., Shimada, M., Yokosawa, H. and Kawahara, H.
Biochem. J. (London)   405(3) 495-501   2007   [Refereed]
Shimada, M., Kanematsu, K., Tanaka, K., Yokosawa, H. and Kawahara, H.
Mol. Biol. Cell   17(12) 5356-5371   Jun 2006   [Refereed]
Stomach-specific calpain localizes in mucus cells and proteolyses the β-subunit of coatomer complex, β-COP.
Hata, S., Koyama, S., Kawahara, H., Doi, N., Maeda, T., Toyama-Sorimachi, N., Abe, K., Suzuki, K., and Sorimachi, H.
J. Biol. Chem.   281 11214-11224   Jan 2006   [Refereed]
Unique proteasome subunit Xrpn10c is a specific receptor for the antiapoptotic ubiquitin-like protein Scythe.
Kikukawa, Y., Minami, R., Shimada, M., Kobayashi, M., Tanaka, K., Yokosawa, H., and Kawahara, H.
FEBS J.   272 6373-6386   Jun 2005   [Refereed]
Knocking out ubiquitin proteasome system function in vivo and in vitro with genetically-encodable tandem ubiquitin.
Saeki, Y., Isono, E., Shimada, M., Kawahara, H., Yokosawa, H., and Toh-e, A.
Methods Enzymol.   399 64-74   Jan 2005   [Refereed]
Parkin binds the Rpn10 subunit of the 26S proteasome with the ubiquitin-like domain.
Sakata, E., Yamaguchi, Y., Kurimoto, E., Kikuchi, J., Yokoyama, S., Yamada, S., Kawahara, H., Yokosawa, H., Hattori, N., Mizuno, Y., Tanaka, K. and Kato. K.
EMBO Rep.   4 301-306   Jun 2003   [Refereed]
Phosphorelay-regulated degradation of the yeast Ssk1p response regulator by the ubiquitin-proteasome system.
Sato, N., Kawahara, H., Toh-e, A. and Maeda, T.
Mol. Cell. Biol.   23 6662-6671   May 2003   [Refereed]
Inhibiting proteasome activity causes over-replication of DNA and blocks entry into mitosis in sea urchin embryos.
Kawahara, H., Philipova, R., Yokosawa, H., Patel, R., Tanaka, K. and Whitaker, M.
J. Cell Sci.   113 2659-2670   Dec 2000   [Refereed]
Kawahara, H., Kasahara, M., Nishiyama, A., Ohsumi, K., Goto, T., Kishimoto, T., Saeki, Y., Yokosawa, H., Shimbara, N., Mutrata, S., Chiba, T., Suzuki, K. and Tanaka, K.
EMBO J.   19 4144-4153   Mar 2000   [Refereed]
Growth retardation of mice lacking the proteasome activator PA28 γ.
Murata, S., Kawahara, H., Tohma, S., Kasahara, K., Tanaka, K. and Chiba, T.
J. Biol. Chem.   274 38211-38215   Jun 1999   [Refereed]


Pre-emptive Protein quality control
Hiroyuki Kawahara
   2019   [Invited]
Ubiquitin-mediated protein quality control and diseases
Hiroyuki Kawahara
The World of UBIQUITIN   3    Dec 2015   [Invited]
35 11-15   May 2008   [Invited]
The ubiquitin receptors.
Kawahara H, Shimada M, Minami R, Yokosawa H.
増刊号 1245-1250   Dec 2006   [Invited]
The ubiquitin recognition domains.
Saeki Y, Kawahara H, Yokosawa H.
21 340-345   Nov 2003   [Invited]

Conference Activities & Talks

BAG6 regulates cytoplasmic pool of Rab family small GTPases [Invited]
Hiroyuki Kawahara
The 41st Annual Meeting of the Molecular Biology Society of Japan   28 Nov 2018   
BAG6 is essential for selective elimination of aggregation-prone defective proteins [Invited]
Hiroyuki Kawahara
35th Naito Conference   22 Jul 2013   
BAG-6/Scythe is essential for selective elimination of defective proteasomal substrates [Invited]
Hiroyuki Kawahara
Dec 2009   

Research Grants & Projects

Project Year: Apr 2017 - Mar 2020    Investigator(s): Hiroyuki Kawahara
Takeda Science Foundation: Specific grant
Project Year: Jul 2019 - Mar 2019    Investigator(s): Hiroyuki Kawahara
Uehara Memorial Foundation: Uehara Grant
Project Year: Apr 2017 - Mar 2019    Investigator(s): Hiroyuki Kawahara
Project Year: Apr 2016 - Mar 2018    Investigator(s): Hiroyuki Kawahara
Project Year: Jun 2011 - Mar 2016    Investigator(s): Hiroyuki Kawahara
Project Year: Apr 2011 - Mar 2014    Investigator(s): Hiroyuki Kawahara
Project Year: Apr 2011 - Mar 2013    Investigator(s): Hiroyuki Kawahara
Naito Foundation: Naito Grant
Project Year: Apr 2011 - Mar 2012    Investigator(s): Hiroyuki Kawahara
Uehara Foundation: Uehara Grant
Project Year: Jan 2011 - Mar 2012    Investigator(s): Hiroyuki Kawahara
Project Year: Jun 2006 - Mar 2011    Investigator(s): Hiroyuki Kawahara

Social Contribution

Editorial Advisory Board, The Journal of Biochemistry, Oxford University Press
[Editor]  Oxford University Press  The Journal of Biochemistry  1 Jan 2014 - 31 Dec 2017