論文

査読有り
2014年6月

Non-destructive hydrocarbon extraction from Botryococcus braunii BOT-22 (race B)

Journal of Applied Phycology
  • Navid R. Moheimani
  • ,
  • Hiroshi Matsuura
  • ,
  • Makoto M. Watanabe
  • ,
  • Michael A. Borowitzka

26
3
開始ページ
1453
終了ページ
1463
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s10811-013-0179-0
出版者・発行元
SPRINGER

There is worldwide interest in developing algal biofuel. One main reason for the lack of success so far in producing a sustainable transport fuel from microalgae is the high cost of biomass processing, especially dewatering and oil extraction. There is also a significant cost involved in the energy content of the nutrient fertilisers required for biomass production. Non-destructive oil extraction or "milking" from algae biomass has the potential to bypass all of these hurdles. Using a "milking" strategy means that there would be no need for (a) biomass dewatering, (b) breaking cells for oil extraction and (c) addition of nutrients to the culture, resulting in a significant reduction in energy and fertiliser cost involved in production of biofuel from algae. We make use of the natural tendency of Botryococcus to produce external hydrocarbon in the extracellular matrix. In current study, we showed that external hydrocarbon from Botryococcus braunii BOT-22 can be non-destructively extracted using n-heptane (optimum contact time with n-heptane = 20 min). We were able to recover almost the entire de novo-produced external hydrocarbons at 5- and 11-day intervals when the culture was maintained with or without 1 % CO2 addition, respectively. This repeated non-destructive extraction of external hydrocarbon of B. braunii was possible for up to 70 days when 1 % CO2 was supplied to the culture. When CO2 was limited, a 70 % lower external hydrocarbon productivity was achieved using the same process. Although the productivity of external hydrocarbon of 9.33 mg L-1 day(-1) of the "milked" culture is low in these un-optimised cultures, it was 1.3 +/- 0.2-fold higher compared with that of a conventional semicontinuous culture, showing the potential of this method.

リンク情報
DOI
https://doi.org/10.1007/s10811-013-0179-0
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000336371400014&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s10811-013-0179-0
  • ISSN : 0921-8971
  • eISSN : 1573-5176
  • Web of Science ID : WOS:000336371400014

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