論文

査読有り 国際誌
2021年10月14日

MORC3, a novel MIWI2 association partner, as an epigenetic regulator of piRNA dependent transposon silencing in male germ cells.

Scientific reports
  • Kanako Kojima-Kita
  • ,
  • Satomi Kuramochi-Miyagawa
  • ,
  • Manabu Nakayama
  • ,
  • Haruhiko Miyata
  • ,
  • Steven E Jacobsen
  • ,
  • Masahito Ikawa
  • ,
  • Haruhiko Koseki
  • ,
  • Toru Nakano

11
1
開始ページ
20472
終了ページ
20472
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-021-98940-7
出版者・発行元
Springer Science and Business Media LLC

<title>Abstract</title>The PIWI (P-element-induced wimpy testis)-interacting-RNA (piRNA) pathway plays a crucial role in the repression of TE (transposable element) expression via de novo DNA methylation in mouse embryonic male germ cells. Various proteins, including MIWI2 are involved in the process. TE silencing is ensured by piRNA-guided MIWI2 that recruits some effector proteins of the DNA methylation machinery to TE regions. However, the molecular mechanism underlying the methylation is complex and has not been fully elucidated. Here, we identified MORC3 as a novel associating partner of MIWI2 and also a nuclear effector of retrotransposon silencing via piRNA-dependent de novo DNA methylation in embryonic testis. Moreover, we show that MORC3 is important for transcription of piRNA precursors and subsequently affects piRNA production. Thus, we provide the first mechanistic insights into the role of this effector protein in the first stage of piRNA biogenesis in embryonic TE silencing mechanism.

リンク情報
DOI
https://doi.org/10.1038/s41598-021-98940-7
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34650118
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516955
URL
https://www.nature.com/articles/s41598-021-98940-7.pdf
URL
https://www.nature.com/articles/s41598-021-98940-7
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85117362665&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85117362665&origin=inward
ID情報
  • DOI : 10.1038/s41598-021-98940-7
  • eISSN : 2045-2322
  • PubMed ID : 34650118
  • PubMed Central 記事ID : PMC8516955
  • SCOPUS ID : 85117362665

エクスポート
BibTeX RIS