2021年10月14日
MORC3, a novel MIWI2 association partner, as an epigenetic regulator of piRNA dependent transposon silencing in male germ cells.
Scientific reports
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- 巻
- 11
- 号
- 1
- 開始ページ
- 20472
- 終了ページ
- 20472
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41598-021-98940-7
- 出版者・発行元
- Springer Science and Business Media LLC
<title>Abstract</title>The PIWI (P-element-induced wimpy testis)-interacting-RNA (piRNA) pathway plays a crucial role in the repression of TE (transposable element) expression via de novo DNA methylation in mouse embryonic male germ cells. Various proteins, including MIWI2 are involved in the process. TE silencing is ensured by piRNA-guided MIWI2 that recruits some effector proteins of the DNA methylation machinery to TE regions. However, the molecular mechanism underlying the methylation is complex and has not been fully elucidated. Here, we identified MORC3 as a novel associating partner of MIWI2 and also a nuclear effector of retrotransposon silencing via piRNA-dependent de novo DNA methylation in embryonic testis. Moreover, we show that MORC3 is important for transcription of piRNA precursors and subsequently affects piRNA production. Thus, we provide the first mechanistic insights into the role of this effector protein in the first stage of piRNA biogenesis in embryonic TE silencing mechanism.
- リンク情報
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- DOI
- https://doi.org/10.1038/s41598-021-98940-7
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/34650118
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8516955
- URL
- https://www.nature.com/articles/s41598-021-98940-7.pdf
- URL
- https://www.nature.com/articles/s41598-021-98940-7
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85117362665&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85117362665&origin=inward
- ID情報
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- DOI : 10.1038/s41598-021-98940-7
- eISSN : 2045-2322
- PubMed ID : 34650118
- PubMed Central 記事ID : PMC8516955
- SCOPUS ID : 85117362665