2022年8月1日
IRGC1, a testis-enriched immunity related GTPase, is important for fibrous sheath integrity and sperm motility in mice.
Developmental biology
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- 巻
- 488
- 号
- 開始ページ
- 104
- 終了ページ
- 113
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.ydbio.2022.05.011
- 出版者・発行元
- Elsevier BV
Immunity-related GTPases (IRGs), also known as p47 GTPases, are a family of interferon-inducible proteins that play roles in immunity defense against intracellular pathogens. Although the molecular functions of IRGs have been well studied, the function of the family member, IRGC1, remains unclear. IRGC1 is unique among IRGs because its expression is not induced by interferon and it is expressed predominantly in the testis. Further, IRGC1 is well conserved in mammals unlike other IRGs. Here, we knocked out (KO) Irgc1 in mice using the CRISPR/Cas9 system and found that the fertility of Irgc1 KO males was severely impaired because of abnormal sperm motility. Further analyses with a transmission electron microscope revealed that the fibrous sheath (FS), an accessory structure of the sperm tail, was disorganized in Irgc1 KO mice. In addition, IRGC1 was detected in the sperm tail and fractionated with FS proteins. These results suggest that IRGC1 is a component of the FS and is involved in the correct formation of the FS.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.ydbio.2022.05.011
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/35618043
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232189
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85131361233&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85131361233&origin=inward
- ID情報
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- DOI : 10.1016/j.ydbio.2022.05.011
- ISSN : 0012-1606
- eISSN : 1095-564X
- PubMed ID : 35618043
- PubMed Central 記事ID : PMC9232189
- SCOPUS ID : 85131361233