2017年12月
Gas-Phase Stability of Negatively Charged Organophosphate Metabolites Produced by Electrospray Ionization and Matrix-Assisted Laser Desorption/Ionization
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
- ,
- ,
- 巻
- 28
- 号
- 12
- 開始ページ
- 2561
- 終了ページ
- 2568
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s13361-017-1795-8
- 出版者・発行元
- SPRINGER
The formation mechanisms of singly and multiply charged organophosphate metabolites by electrospray ionization (ESI) and their gas phase stabilities were investigated. Metabolites containing multiple phosphate groups, such as adenosine 5'-diphosphate (ADP), adenosine 5'-triphosphate (ATP), and D-myo-inositol-1,4,5-triphosphate (IP3) were observed as doubly deprotonated ions by negative-ion ESI mass spectrometry. Organophosphates with multiple negative charges were found to be unstable and often underwent loss of PO3 (-), although singly deprotonated analytes were stable. The presence of fragments due to the loss of PO3 (-) in the negative-ion ESI mass spectra could result in the misinterpretation of analytical results. In contrast to ESI, matrix-assisted laser desorption ionization (MALDI) produced singly charged organophosphate metabolites with no associated fragmentation, since the singly charged anions are stable. The stability of an organophosphate metabolite in the gas phase strongly depends on its charge state. The fragmentations of multiply charged organophosphates were also investigated in detail through density functional theory calculations.
- リンク情報
- ID情報
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- DOI : 10.1007/s13361-017-1795-8
- ISSN : 1044-0305
- eISSN : 1879-1123
- PubMed ID : 28875264
- Web of Science ID : WOS:000416567600005