2018年6月20日
A mitochondria-targeted fluorescent probe for selective detection of mitochondrial labile Fe(ii).
Metallomics : integrated biometal science
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- ,
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- 巻
- 10
- 号
- 6
- 開始ページ
- 794
- 終了ページ
- 801
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1039/c8mt00049b
Mitochondria are iron-rich organelles that are involved in the process of energy production through the electron-transporting system and heme synthesis. We developed a new mitochondria-targeted fluorescent probe, MtFluNox/Ac-MtFluNox, for Fe(ii) based on N-oxide chemistry, which we recently established as a Fe(ii)-selective fluorogenic switch. The deacetylated form MtFluNox showed a turn-on response towards Fe(ii) with high metal selectivity in cuvette experiments, and an imaging study using its cell-compatible analogue Ac-MtFluNox demonstrated mitochondria-specific fluorescence enhancement in response to Fe(ii) in living cells. Furthermore, the probe was able to detect endogenously accumulated Fe(ii) induced as a result of the inhibition of heme synthesis.
- ID情報
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- DOI : 10.1039/c8mt00049b
- PubMed ID : 29863204