2018年6月
Reciprocal regulation of TEAD4 and CCN2 for the trophectoderm development of the bovine blastocyst
Reproduction
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- 巻
- 155
- 号
- 6
- 開始ページ
- 563
- 終了ページ
- 571
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1530/rep-18-0043
- 出版者・発行元
- Bioscientifica
The first segregation at the blastocyst stage is the symmetry-breaking event to characterize two cell components; namely, inner cell mass (ICM) and trophectoderm (TE). TEA domain transcription factor 4 (TEAD4) is a well-known regulator to determine TE properties of blastomeres in rodent models. However, the roles of bovine TEAD4 in blastocyst development have been unclear. We here aimed to clarify the mechanisms underlining TE characterization by TEAD4 in bovine blastocysts. We first found that the<italic>TEAD4</italic>mRNA expression level was greater in TE than in ICM, which was further supported by TEAD4 immunofluorescent staining. Subsequently, we examined the expression patterns of TE-expressed genes;<italic>CDX2</italic>,<italic>GATA2</italic>and<italic>CCN2</italic>, in the<italic>TEAD4</italic>-knockdown (KD) blastocysts. These expression levels significantly decreased in the<italic>TEAD4</italic>KD blastocysts compared with controls. Of these downregulated genes, the<italic>CCN2</italic>expression level decreased the most. We further analyzed the expression levels of TE-expressed genes;<italic>CDX2</italic>,<italic>GATA2</italic>and<italic>TEAD4</italic>in the<italic>CCN2</italic>KD blastocysts. Strikingly, the<italic>CCN2</italic>KD blastocysts showed the downregulation of<italic>CDX2</italic>,<italic>GATA2</italic>and<italic>TEAD4</italic>. Furthermore, the ratio of TE-to-ICM cell numbers in the<italic>CCN2</italic>KD blastocysts significantly decreased compared to controls. To our knowledge, this is the first study showing the regulation of<italic>CCN2</italic>expression thorough<italic>TEAD4</italic>in mammalian embryos. Not only that, this study also provides evidence that reciprocal regulation of<italic>TEAD4</italic>and<italic>CCN2</italic>is required for TE development with appropriate gene expression in bovine blastocysts.
- リンク情報
- ID情報
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- DOI : 10.1530/rep-18-0043
- ISSN : 1470-1626
- eISSN : 1741-7899
- PubMed ID : 29661794