論文

査読有り
2009年10月

Design of a bioreductively-activated fluorescent pH probe for tumor hypoxia imaging

BIOORGANIC & MEDICINAL CHEMISTRY
  • Eiji Nakata
  • ,
  • Yoshihiro Yukimachi
  • ,
  • Hirokazu Kariyazono
  • ,
  • Seongwang Im
  • ,
  • Chiaki Abe
  • ,
  • Yoshihiro Uto
  • ,
  • Hiroshi Maezawa
  • ,
  • Toshihiro Hashimoto
  • ,
  • Yasuko Okamoto
  • ,
  • Hitoshi Hori

17
19
開始ページ
6952
終了ページ
6958
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bmc.2009.08.037
出版者・発行元
PERGAMON-ELSEVIER SCIENCE LTD

We have designed and evaluated UTX-12 as a novel fluorescent pH probe for tumor hypoxia imaging. UTX-12 consists of a p-nitro benzyl moiety, which is a latent hypoxia-selective leaving group activated by nitro reduction, directly linked to SNARF. Although UTX-12 itself is colorless and non-fluorescent in aqueous solution, nitro reduction triggers the release of SNARF which has well-characterized long wavelength absorption and fluorescence that is sensitive to pH. The resultant SNARF, released intracellularly by enzymatic reduction of UTX-12, allows measurement of pH by pH-dependent dual emission shifts. UTX-12 showed clear differences in fluorescence behavior between hypoxic and aerobic conditions in liver microsomes and inside V79 cells. These data are confirmation that UTX-12 is biologically reduced inside tumor cells and the released SNARF should monitor intracellular pH of tumor cells selectively with reduced background signal. (C) 2009 Elsevier Ltd. All rights reserved.

Web of Science ® 被引用回数 : 67

リンク情報
DOI
https://doi.org/10.1016/j.bmc.2009.08.037
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19736018
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000269724600019&DestApp=WOS_CPL