1996年9月
Promoter-independent loss of mRNA and protein of the Rb gene in a human hepatocellular carcinoma
HEPATO-GASTROENTEROLOGY
- 巻
- 43
- 号
- 11
- 開始ページ
- 1185
- 終了ページ
- 1189
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- H G E UPDATE MEDICAL PUBL LTD.
Background: Inactivation of the retinoblastoma (Rb) gene is considered to play a fundamental role in the genesis and progression of several human cancers. In retinoblastoma, the inactivation of Rb promoter by mutations or hypermethylation has been reported. Although genetic changes of Rb gene have been described in hepatocellular carcinoma (HCC) an, epigenetic change such as hypermethylation of the Rb promoter as reported in, retinoblastoma has not been described.
Materials and Methods: We examined the hypermethylation in the promoter region of Rb gene by restriction fragment length polymorphism in 19 HCCs, as well as the expression of Rb mRNA and protein by RT-PCR and by immunoblotting, respectively.
Results: We found no evidence of hypermethylation. in the promoter region, of the Rb gene in all HCCs analyzed. However, the expression of Rb mRNA and protein was lost in one HCC, and no mutation was detected in the Rb promoter region of this patient. The inactivation of Rb promoter by hypermethylation or by inhibition of binding of transcription factors due to point mutations did not contribute to the loss of mRNA and protein in. the patient.
Conclusions: Hypermethylation in the Rb promoter region appeared to have little causal effect on HCC.
Materials and Methods: We examined the hypermethylation in the promoter region of Rb gene by restriction fragment length polymorphism in 19 HCCs, as well as the expression of Rb mRNA and protein by RT-PCR and by immunoblotting, respectively.
Results: We found no evidence of hypermethylation. in the promoter region, of the Rb gene in all HCCs analyzed. However, the expression of Rb mRNA and protein was lost in one HCC, and no mutation was detected in the Rb promoter region of this patient. The inactivation of Rb promoter by hypermethylation or by inhibition of binding of transcription factors due to point mutations did not contribute to the loss of mRNA and protein in. the patient.
Conclusions: Hypermethylation in the Rb promoter region appeared to have little causal effect on HCC.
- リンク情報
- ID情報
-
- ISSN : 0172-6390
- Web of Science ID : WOS:A1996VP41800019