論文

国際誌
2019年10月15日

Characterization of fructooligosaccharide-degrading enzymes in human commensal Bifidobacterium longum and Anaerostipes caccae

Biochemical and Biophysical Research Communications
  • Hiroki Tanno
  • ,
  • Tadashi Fujii
  • ,
  • Riichi Ose
  • ,
  • Katsuaki Hirano
  • ,
  • Takumi Tochio
  • ,
  • Akihito Endo

518
2
開始ページ
294
終了ページ
298
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2019.08.049

Kestose and nystose are short chain fructooligosaccharides (scFOSs) with degrees of polymerization of 3 and 4, respectively. A previous study revealed that these scFOSs have different growth stimulation properties against two human commensals, i.e. Bifidobacterium longum subsp. longum and butyrogenic Anaerostipes caccae. The present study characterized genes involved in FOS metabolism in these organisms. A. caccae possesses a single gene cluster consisting of four genes, including a gene encoding the putative FOS degradation enzyme sucrose-6-phosphate hydrolase (S6PH). B. longum possesses two gene clusters consisting of three genes each, including genes encoding β-fructofuranosidase (CscA) and sucrose phosphorylase (ScrP). In A. caccae, the genes were highly transcribed in cells cultured with sucrose or kestose but poorly in cells cultured with glucose or nystose. Heterologously expressed S6PH degraded sucrose and kestose but not nystose. In B. longum, transcription of the genes was high in cells cultured with sucrose or kestose but was poor or not detected in cells cultured with glucose or nystose. Heterologously expressed CscA degraded sucrose, kestose and nystose, but ScrP degraded only sucrose. These data suggested that the different growth stimulation activities of kestose and nystose are due to different substrate specificities of FOS degradation enzymes in the organisms and/or induction activity of the genes in the two scFOSs. This is the first study characterizing the FOS metabolism at the transcriptional level and substrate-specificity of the degradation enzyme in butyrogenic human gut anaerobes.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2019.08.049
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31420164
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85070486886&origin=inward
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85070486886&origin=inward
ID情報
  • DOI : 10.1016/j.bbrc.2019.08.049
  • ISSN : 0006-291X
  • eISSN : 1090-2104
  • PubMed ID : 31420164
  • SCOPUS ID : 85070486886

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