論文

査読有り 国際誌
2012年6月

A protein transduction method using oligo-arginine (3R) for the delivery of transcription factors into cell nuclei

Biomaterials
  • Takashi Hitsuda
  • Hiroyuki Michiue
  • Mizuki Kitamatsu
  • Atsushi Fujimura
  • Feifei Wang
  • Takahiro Yamamoto
  • Xiao-Jian Han
  • Hiroshi Tazawa
  • Atsuhito Uneda
  • Iori Ohmori
  • Tei-ichi Nishiki
  • Kazuhito Tomizawa
  • Hideki Matsui
  • 全て表示

33
18
開始ページ
4665
終了ページ
4672
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.biomaterials.2012.02.049
出版者・発行元
ELSEVIER SCI LTD

Protein transduction with cell-penetrating peptides such as poly-arginine and HIV TAT peptides is widely used to deliver proteins, peptides, siRNA and biologically active compounds. It has been thought that poly-arginine peptides transduce proteins in a manner dependent on the number of arginine residues and oligo-peptides such as three arginines (3R) are ineffective. Here we showed that 3R-fused proteins were effectively delivered and functioned in cells co-treated with pyrenebutyrate, a counteranion bearing an aromatic hydrophobic moiety. Little 3R was transduced in glioma cells without pyrenebutyrate whereas the oligo-arginine was effectively delivered with pyrenebutyrate. Enhanced green fluorescence protein (eGFP) fused with 3R was effectively delivered into various kinds of cells including primary cultured cells and suspended cells in the presence of pyrenebutyrate. p53 fused with 3R (3R-p53) was delivered into glioma cells without pyrenebutyrate but could not be translocated into the ncleus. In contrast, 3R-p53 was observed in nuclei of glioma cells when co-applied with pyrenebutyrate. Although 3R-p53 was delivered less effectively than 11R-p53 with pyrenebutyrate, its transcriptional activity was higher than that of 11R-p53. Moreover, a single administration of 3R-p53 with pyrenebutyrate significantly inhibited the growth of cancer cells. These results suggest protein transduction using an oligo-arginine (3R) with pyrenebutyrate to be a good tool for the delivery of functional transcription factors and a promising method of treating cancer. (C) 2012 Elsevier Ltd. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.biomaterials.2012.02.049
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000303953000024&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.biomaterials.2012.02.049
  • ISSN : 0142-9612
  • eISSN : 1878-5905
  • Web of Science ID : WOS:000303953000024

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