2014年11月
From fluorescence polarization to Quenchbody: Recent progress in fluorescent reagentless biosensors based on antibody and other binding proteins
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
- ,
- 巻
- 1844
- 号
- 11
- 開始ページ
- 1951
- 終了ページ
- 1959
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.bbapap.2014.06.005
- 出版者・発行元
- ELSEVIER SCIENCE BV
Recently, antibody-based fluorescent biosensors are receiving considerable attention as a suitable biomolecule for diagnostics, namely, homogeneous immunoassay and also as an imaging probe. To date, several strategies for "reagentless biosensors" based on antibodies and natural and engineered binding proteins have been described. In this review, several approaches are introduced including a recently described fluorescent antibody-based biosensor Quenchbody, which works on the principle of fluorescence quenching of attached dye and its antigen-dependent release. The merits and possible demerits of each approach are discussed. This article is part of a Special Issue entitled: Recent advances in molecular engineering of antibody. (C) 2014 Elsevier B.V. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.bbapap.2014.06.005
- ISSN : 1570-9639
- eISSN : 0006-3002
- ORCIDのPut Code : 15680215
- Web of Science ID : WOS:000343624200008