2004年12月
BIG2, a guanine nucleotide exchange factor for ADP-ribosylation factors: Its localization to recycling endosomes and implication in the endosome integrity
MOLECULAR BIOLOGY OF THE CELL
- ,
- ,
- ,
- 巻
- 15
- 号
- 12
- 開始ページ
- 5283
- 終了ページ
- 5294
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1091/mbc.E04-05-0388
- 出版者・発行元
- AMER SOC CELL BIOLOGY
Small GTPases of the ADP-ribosylation factor (ARF) family play a key role in membrane trafficking by regulating coated vesicle formation, and guanine nucleotide exchange is essential for the ARF function. Brefeldin A blocks the ARF-triggered coat assembly by inhibiting the guanine nucleotide exchange on ARFs and causes disintegration of the Golgi complex and tubulation of endosomal membranes. BIG2 is one of brefeldin A-inhibited guanine nucleotide exchange factors for the ARF GTPases and is associated mainly with the trans-Golgi network. In the present study, we have revealed that another population of BIG2 is associated with the recycling endosome and found that expression of a catalytically inactive BIG2 mutant, E738K, selectively induces membrane tubules from this compartment. We also have shown that BIG2 has an exchange activity toward class I ARFs (ARF1 and ARF3) in vivo and inactivation of either ARF exaggerates the BIG2(E738K)-induced tubulation of endosomal membranes. These observations together indicate that BIG2 is implicated in the structural integrity of the recycling endosome through activating class I ARFs.
- リンク情報
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- DOI
- https://doi.org/10.1091/mbc.E04-05-0388
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/15385626
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000225372800008&DestApp=WOS_CPL
- URL
- http://europepmc.org/articles/PMC532010
- URL
- http://orcid.org/0000-0002-9138-9554
- ID情報
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- DOI : 10.1091/mbc.E04-05-0388
- ISSN : 1059-1524
- ORCIDのPut Code : 52022432
- PubMed ID : 15385626
- Web of Science ID : WOS:000225372800008