論文

査読有り
2017年8月

Diversity and community structure of wood-inhabiting fungi found in Japanese wooden houses analyzed by the next-generation sequencing

JOURNAL OF WOOD SCIENCE
  • Sakae Horisawa
  • ,
  • Makoto Yoshida
  • ,
  • Kiwamu Umezawa
  • ,
  • Tomoko Wada
  • ,
  • Hisashi Abe
  • ,
  • Shuichi Doi
  • ,
  • Masahiro Samejima
  • ,
  • Ikuo Momohara

63
4
開始ページ
369
終了ページ
378
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s10086-017-1630-7
出版者・発行元
SPRINGER JAPAN KK

The diversity and community structures of wood-inhabiting fungi in 16 decayed wood samples from ten wooden houses in Japan were analyzed using a next-generation sequencing (NGS) to determine the fungi responsible for wood decay. DNA of fungi in decayed wood was extracted directly, the internal transcribed spacer (ITS) region in ribosomal DNA (rDNA) was amplified by polymerase chain reaction (PCR), and then, sequences of tagged ITS fragments were analyzed by NGS. Results of sequencing indicated that 68 species of ascomycetes, 37 species of basidiomycetes, and one fungus each from Mortierellales and Mucoromycetes were detected. The fungal community structures showed diversity and included various species of ascomycetes. A microscopic examination of cell wall structure in decayed wood samples suggested that some ascomycetes were soft-rot fungi. Heat map analysis indicated that the similarity in the structures of fungal communities was influenced to a greater extent by the wood species of samples than where they were used as a component.


リンク情報
DOI
https://doi.org/10.1007/s10086-017-1630-7
J-GLOBAL
https://jglobal.jst.go.jp/detail?JGLOBAL_ID=201702257755844181
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000406185400007&DestApp=WOS_CPL
URL
http://jglobal.jst.go.jp/public/201702257755844181
ID情報
  • DOI : 10.1007/s10086-017-1630-7
  • ISSN : 1435-0211
  • eISSN : 1611-4663
  • J-Global ID : 201702257755844181
  • Web of Science ID : WOS:000406185400007

エクスポート
BibTeX RIS