Jul 21, 2006
Lysenin forms a voltage-dependent channel in artificial lipid bilayer membranes.
Biochemical and biophysical research communications
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- Volume
- 346
- Number
- 1
- First page
- 288
- Last page
- 92
- Language
- English
- Publishing type
- Research paper (scientific journal)
Lysenin, a hemolytic protein derived from the body fluid of earthworm, was incorporated into artificial bilayer membranes. Upon insertion, it formed a voltage-dependent large conductance channel in asolectin bilayers in a sphingomyelin-dependent manner. The channel had low ion-selectivity. Single-channel conductance was calculated as approximately 550 pS in 100 mM KCl. The channel in asolectin bilayers closed when the membrane was held at a positive potential. In contrast, the channel showed no voltage dependency in membranes made of pure phosphatidylcholine and sphingomyelin, suggesting some lipid contents included in the asolectin membranes affected channel gating.
- Link information
- ID information
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- ISSN : 0006-291X
- Pubmed ID : 16756950