2021年4月29日
N-terminally endogenous mKate2-tagged HIM-4 in Caenorhabditis elegans.
microPublication biology
- 巻
- 2021
- 号
- 開始ページ
- Open access
- 終了ページ
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.17912/micropub.biology.000386
The gene him-4 encodes the C. elegans homolog of hemicentin, an evolutionarily conserved extracellular matrix protein. Despite being an extracellular matrix protein, mutations in him-4 result in pleiotropic defects during development and demonstrate tissue fragility. While previous studies in C. elegans have confirmed the localization of either transgenic HIM-4::GFP or endogenous C-terminally tagged HIM-4::mNeonGreen, only green or green/yellow fluorescent protein-tagged HIM-4 are available to the C. elegans community. Here, I used CRISPR/Cas9 technology to insert the far-red fluorescence protein mKate2 at the him-4 genomic locus, and established worms expressing mKate2::HIM-4. As expected, localization of mkate2::HIM-4 at the rachis was observed at the L4 stage. In contrast to the localization of type IV collagen or nidogen such as major components of the basement membrane, mkate2::HIM-4 was polarized at the anterior part of the pharyngeal basement membrane. A unique polarized localization pattern of pharyngeal basement membrane is maintained throughout the L1-L4 stages.
- リンク情報
- ID情報
-
- DOI : 10.17912/micropub.biology.000386
- PubMed ID : 33937723
- PubMed Central 記事ID : PMC8085682