論文

査読有り 国際誌
2019年1月18日

Cellular cartography of the organ of Corti based on optical tissue clearing and machine learning.

eLife
  • Shinji Urata
  • ,
  • Tadatsune Iida
  • ,
  • Masamichi Yamamoto
  • ,
  • Yu Mizushima
  • ,
  • Chisato Fujimoto
  • ,
  • Yu Matsumoto
  • ,
  • Tatsuya Yamasoba
  • ,
  • Shigeo Okabe

8
記述言語
英語
掲載種別
DOI
10.7554/eLife.40946

The highly organized spatial arrangement of sensory hair cells in the organ of Corti is essential for inner ear function. Here, we report a new analytical pipeline, based on optical clearing of tissue, for the construction of a single-cell resolution map of the organ of Corti. A sorbitol-based optical clearing method enabled imaging of the entire cochlea at subcellular resolution. High-fidelity detection and analysis of all hair cell positions along the entire longitudinal axis of the organ of Corti were performed automatically by machine learning-based pattern recognition. Application of this method to samples from young, adult, and noise-exposed mice extracted essential information regarding cellular pathology, including longitudinal and radial spatial characteristics of cell loss, implying that multiple mechanisms underlie clustered cell loss. Our method of cellular mapping is effective for system-level phenotyping of the organ of Corti under both physiological and pathological conditions.

リンク情報
DOI
https://doi.org/10.7554/eLife.40946
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30657453
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338463

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