論文

査読有り
2016年5月

Fast 3D visualization of endogenous brain signals with high-sensitivity laser scanning photothermal microscopy

BIOMEDICAL OPTICS EXPRESS
  • Jun Miyazaki
  • ,
  • Tadatsune Iida
  • ,
  • Shinji Tanaka
  • ,
  • Akiko Hayashi-Takagi
  • ,
  • Haruo Kasai
  • ,
  • Shigeo Okabe
  • ,
  • Takayoshi Kobayashi

7
5
開始ページ
1702
終了ページ
1710
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1364/BOE.7.001702
出版者・発行元
OPTICAL SOC AMER

A fast, high-sensitivity photothermal microscope was developed by implementing a spatially segmented balanced detection scheme into a laser scanning microscope. We confirmed a 4.9 times improvement in signal-to-noise ratio in the spatially segmented balanced detection compared with that of conventional detection. The system demonstrated simultaneous bi-modal photothermal and confocal fluorescence imaging of transgenic mouse brain tissue with a pixel dwell time of 20 mu s. The fluorescence image visualized neurons expressing yellow fluorescence proteins, while the photothermal signal detected endogenous chromophores in the mouse brain, allowing 3D visualization of the distribution of various features such as blood cells and fine structures probably due to lipids. This imaging modality was constructed using compact and cost-effective laser diodes, and will thus be widely useful in the life and medical sciences. (C) 2016 Optical Society of America

Web of Science ® 被引用回数 : 10

リンク情報
DOI
https://doi.org/10.1364/BOE.7.001702
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27231615
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000374745200007&DestApp=WOS_CPL

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