論文

査読有り
2014年2月

Intracellular Propionibacterium acnes Infection in Glandular Epithelium and Stromal Macrophages of the Prostate with or without Cancer

PLOS ONE
  • Yuan Bae
  • ,
  • Takashi Ito
  • ,
  • Tadatsune Iida
  • ,
  • Keisuke Uchida
  • ,
  • Masaki Sekine
  • ,
  • Yutaka Nakajima
  • ,
  • Jiro Kumagai
  • ,
  • Tetsuji Yokoyama
  • ,
  • Hiroshi Kawachi
  • ,
  • Takumi Akashi
  • ,
  • Yoshinobu Eishi

9
2
開始ページ
e90324
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0090324
出版者・発行元
PUBLIC LIBRARY SCIENCE

Background: Recent reports on Propionibacterium acnes (P. acnes) suggest that this bacterium is prevalent in the prostate, is associated with acute and chronic prostatic inflammation, and might have a role in prostate carcinogenesis.
Methods: To evaluate the pathogenic role of this indigenous bacterium, we screened for the bacterium in radical prostatectomy specimens using enzyme immunohistochemistry with a novel P. acnes-specific monoclonal antibody (PAL antibody), together with an anti-nuclear factor-kappa B (NF-kappa B) antibody. We examined formalin-fixed and paraffin-embedded tissue sections of radical prostatectomy specimens from 28 patients with prostate cancer and 18 age-matched control patients with bladder cancer, but without prostate cancer.
Results: Immunohistochemistry with the PAL antibody revealed small round bodies within some non-cancerous glandular epithelium and stromal macrophages in most prostate samples. Prostate cancer samples had higher frequencies of either cytoplasmic P. acnes or nuclear NF-kappa B expression of glandular epithelium and higher numbers of stromal macrophages with P. acnes than control samples. These parameters were also higher in the peripheral zone than in the transitional zone of the prostate, especially in prostate cancer samples. Nuclear NF-kappa B expression was more frequent in glands with P. acnes than in glands without P. acnes. The number of stromal macrophages with the bacterium correlated with the grade of chronic inflammation in both the PZ and TZ areas and with the grade of acute inflammation in the TZ area.
Conclusions: Immunohistochemical analysis with a novel monoclonal antibody for detecting P. acnes in the prostate suggested that intraepithelial P. acnes infection in non-cancerous prostate glands and inflammation caused by the bacterium may contribute to the development of prostate cancer.

Web of Science ® 被引用回数 : 22

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0090324
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24587325
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000332396200171&DestApp=WOS_CPL

エクスポート
BibTeX RIS