論文

査読有り
2007年4月

Ras-association domain family protein 6 induces apoptosis via both caspase-dependent and caspase-independent pathways

EXPERIMENTAL CELL RESEARCH
  • Mitsunobu Ikeda
  • ,
  • Susumu Hirabayashi
  • ,
  • Naoyuki Fujiwara
  • ,
  • Hiroki Mori
  • ,
  • Akira Kawata
  • ,
  • Junko Iida
  • ,
  • Yijun Bao
  • ,
  • Tadatsune Iida
  • ,
  • Haruhiko Sugimura
  • ,
  • Yutaka Hata

313
7
開始ページ
1484
終了ページ
1495
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.yexcr.2007.02.013
出版者・発行元
ELSEVIER INC

The Ras-association domain family (RASSF) comprises six members (RASSF1-6) that each harbors a RalGDS/AF-6 (RA) and Sav/RASSF/Hippo (SARAH) domain. The RASSF proteins are known as putative tumor suppressors but RASSF6 has not yet been studied. We have here characterized human RASSF6. Although RASSF6 has RA domain, it does not bind Ki-Ras, Ha-Ras, N-Ras, M-Ras, or TC21 under the condition that Nore1 (RASSF5) binds these Ras proteins. The message of RASSF6 is detected by RT-PCR in several cell lines including HeLa, MCF-7, U373, A549, and HepG2 cells, but the protein expression is low. The enhanced expression of RASSF6 causes apoptosis in HeLa cells. RASSF6 activates Bax and induces cytochrome C release. Caspase-3 activation is also induced, but the caspase inhibitor, Z-VAD-FMK, does not block RASSF6-mediated apoptosis. Apoptosis-inducing factor and endonuclease G are released from the mitochondria upon expression of RASSF6 and their releases are not blocked by Z-VAD-FMK. The knock down of RASSF6 partially blocks tumor necrosis factor-alpha-induced cell death in HeLa cells. These findings indicate that RASSF6 is implicated in apoptosis in HeLa cells and that it triggers both caspase-dependent and caspase-independent pathways. (c) 2007 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 59

リンク情報
DOI
https://doi.org/10.1016/j.yexcr.2007.02.013
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17367779
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000245681800018&DestApp=WOS_CPL

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