Ikuko Fujiwara

Profile Information

Affiliation: Assoc. Prof. (PhD), Graduate School of Engineering, Department of Materials Sciences and Bioengineering, Nagaoka University of Technology

Degree: 博士(理学)(早稲田大学)

Researcher number: 10742075
J-GLOBAL ID: 201401039001794673
researchmap Member ID: 7000008066

External link: http://bio.nagaokaut.ac.jp/~honda-l/

アクチンやMreB、それらと相互作用するタンパク質の結合・解離がどのように細胞の運動機能を制御しているのかを、TIRFを主とした蛍光顕微鏡観察で調べています

Research Interests

Research Areas

Research History

Apr, 2022 - Present

Assoc. Prof. (PhD), Graduate School of Engineering, Department of Materials Sciences and Bioengineering, Nagaoka University of Technology
Apr, 2021 - Mar, 2022

Assoc. Prof. (PhD), Graduate School of Engineering Department of Bioengineering, Nagaoka University of Technology
Apr, 2019 - Mar, 2021

Assistant Professor, Graduate School of Science Biology and Geosciences Course, Osaka City University
Jan, 2015 - Mar, 2019

University Research Administrator (Research Assistant Professor), Reasearch Admistration Office, Nagoya Institute of Technology
Apr, 2014 - Dec, 2014

Assistant Professor, Institute for Integrated Cell-Material Sciences, Kyoto University
Sep, 2006 - Mar, 2014

Researcher, National Institutes of Health, USA
Aug, 2004 - Sep, 2006

Researcher, Molecular Cellular and Developmental Biology, Yale University
University Research Administrator (Research Assistant Professor), Nagoya Institute of Technology Reasearch Admistration Office

Education

Apr, 1998 - Mar, 2003

Dept. of Physics, Graduate School, Division of Science and Engineering, Graduate school of science, Waseda University, Japan
- Mar, 1998

Dept of Applied Physics, Faculty of Science and Engineering, Waseda University

Committee Memberships

Jan, 2022 - Dec, 2024

Editorial board member, The Biophysical Society of Japan
Jan, 2021 - Dec, 2021

分野別専門委員 (アクチン), Biophysical society of Japan
Jan, 2020 - Dec, 2020

分野別専門委員 (細胞骨格), Biophysical society of Japan
Jan, 2018 - Dec, 2018

名古屋工業大学ダイバーシティ推進センタースーパーバイザー
Nov, 2016 - Nov, 2017

American Society For Cell Biology (ASCB) 名古屋工業大学アンバサダー

Awards

Oct, 2018

Recommended in F1000Prime as being of special significance in its field by F1000, Latrunculin A accelerates actin filament depolymerization in addition to sequestering actin monomers, Faculty of 1000 Ltd | Science Navigation Group

Fujiwara I, Zweifel ME, Courtemanche N, Pollard TD

Papers

A highly active bacterial actin actuates the polymerization of another isoform essential for swimming motility ofSpiroplasma

Daichi Takahashi, Hana Kiyama, Hideaki Matsubayashi, Ikuko Fujiwara, Makoto Miyata

Sep 8, 2024 Corresponding author
Assembly properties of bacterial actin MreB involved in Spiroplasma swimming motility.

Daichi Takahashi, Makoto Miyata, Ikuko Fujiwara

The Journal of biological chemistry, 299(6) 104793-104793, Jun, 2023 Corresponding author
Phase separation of an actin nucleator by junctional microtubules regulates epithelial function.

Kazuto Tsukita, Manabu Kitamata, Hiroka Kashihara, Tomoki Yano, Ikuko Fujiwara, Timothy F Day, Tatsuya Katsuno, Jaewon Kim, Fumiko Takenaga, Hiroo Tanaka …

Science advances, 9(7) eadf6358, Feb 15, 2023
Purification and ATPase Activity Measurement of Spiroplasma MreB.

Daichi Takahashi, Ikuko Fujiwara, Makoto Miyata

Methods in molecular biology (Clifton, N.J.), 2646 359-371, 2023 Corresponding author
Structures and mechanisms of actin ATP hydrolysis.

Yusuke Kanematsu, Akihiro Narita, Toshiro Oda, Ryotaro Koike, Motonori Ota, Yu Takano, Kei Moritsugu, Ikuko Fujiwara, Kotaro Tanaka, Hideyuki Komatsu …

Proceedings of the National Academy of Sciences of the United States of America, 119(43) e2122641119, Oct 25, 2022
ATP-dependent polymerization dynamics of bacterial actin proteins involved in Spiroplasma swimming.

Daichi Takahashi, Ikuko Fujiwara, Yuya Sasajima, Akihiro Narita, Katsumi Imada, Makoto Miyata

Open biology, 12(10) 220083-220083, Oct, 2022 Corresponding author
Movements of Mycoplasma mobile Gliding Machinery Detected by High-Speed Atomic Force Microscopy.

Kohei Kobayashi, Noriyuki Kodera, Taishi Kasai, Yuhei O Tahara, Takuma Toyonaga, Masaki Mizutani, Ikuko Fujiwara, Toshio Ando, Makoto Miyata

mBio, 12(3) e0004021, Jun 29, 2021
Structural insights into the regulation of actin capping protein by twinfilin C-terminal tail.

Shuichi Takeda, Ryotaro Koike, Ikuko Fujiwara, Akihiro Narita, Makoto Miyata, Motonori Ota, Yuichiro Maéda

Journal of molecular biology, 433(9) 166891-166891, Feb 24, 2021
Crystal structure of human V-1 in the apo form.

Shuichi Takeda, Ryotaro Koike, Takayuki Nagae, Ikuko Fujiwara, Akihiro Narita, Yuichiro Maéda, Motonori Ota

Acta crystallographica. Section F, Structural biology communications, 77(Pt 1) 13-21, Jan 1, 2021
Myosin 18Aα targets the guanine nucleotide exchange factor β-Pix to the dendritic spines of cerebellar Purkinje neurons and promotes spine maturation.

Christopher J Alexander, Melanie Barzik, Ikuko Fujiwara, Kirsten Remmert, Ya-Xian Wang, Ronald S Petralia, Thomas B Friedman, John A Hammer

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(1) e21092, Jan, 2021 Peer-reviewed
Phylogenetic origin and sequence features of MreB from the wall-less swimming bacteria Spiroplasma.

Daichi Takahashi, Ikuko Fujiwara, Makoto Miyata

Biochemical and biophysical research communications, 533(4) 638-644, Dec 17, 2020 Peer-reviewed
D-Loop Mutation G42A/G46A Decreases Actin Dynamics

Mizuki Matsuzaki, Ikuko Fujiwara, Sae Kashima, Tomoharu Matsumoto, Toshiro Oda, Masahito Hayashi, Kayo Maeda, Kingo Takiguchi, Yuichiro Maéda, Akihiro Narita

Biomolecules, 10(5) 736-736, May 8, 2020 Peer-reviewed
Novel inter-domain Ca2+-binding site in the gelsolin superfamily protein fragmin

Shuichi Takeda, Ikuko Fujiwara, Yasunobu Sugimoto, Toshiro Oda, Akihiro Narita, Yuichiro Maéda

Journal of Muscle Research and Cell Motility, 41(1) 153-162, Mar, 2020 Peer-reviewed
Latrunculin A Depolymerizes Actin Filaments by Binding Terminal Subunits and by Sequestering Actin Monomers

Ikuko FUJIWARA

Seibutsu Butsuri, 59(4) 192-196, 2019 Peer-reviewed
Polymerization and depolymerization of actin with nucleotide states at filament ends

Ikuko Fujiwara, Shuichi Takeda, Toshiro Oda, Hajime Honda, Akihiro Narita, Yuichiro Maéda

Biophysical Reviews, 10(6) 1513-1519, Dec, 2018 Peer-reviewedCorresponding author
Latrunculin A Accelerates Actin Filament Depolymerization in Addition to Sequestering Actin Monomers

Fujiwara I, Zweifel ME, Courtemanche N, Pollard TD

Current Biology, 28(19) 3183-3192, Sep 27, 2018 Peer-reviewed
Keeping the focus on biophysics and actin filaments in Nagoya: A report of the 2016 "now in actin" symposium

Ikuko Fujiwara, Akihiro Narita

CYTOSKELETON, 74(12) 450-464, Dec, 2017 Peer-reviewedInvited
Capping protein regulatory cycle driven by CARMIL and V-1 may promote actin network assembly at protruding edges

Ikuko Fujiwara, Kirsten Remmert, Grzegorz Piszczek, John A. Hammer

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(19) E1970-E1979, May, 2014 Peer-reviewed
Morphology and Viscoelasticity of Actin Networks Formed with the Mutually Interacting Crosslinkers: Palladin and Alpha-actinin

Brian Grooman, Ikuko Fujiwara, Carol Otey, Arpita Upadhyaya

PLOS ONE, 7(8) e42773, Aug, 2012
Structural Basis for Capping Protein Sequestration by Myotrophin (V-1)

Adam Zwolak, Ikuko Fujiwara, John A. Hammer, Nico Tjandra

JOURNAL OF BIOLOGICAL CHEMISTRY, 285(33) 25767-25781, Aug, 2010 Peer-reviewed

Misc.

F-form actin structure at an atomic resolution: how the molecular assembly and the ATP hydrolysis are coupled

武田修一, 兼松佑典, 成田哲博, 田中康太郎, 小池亮太郎, 太田元規, 鷹野優, 森次圭, 藤原郁子, 渡邉信久 …

日本物理学会講演概要集(CD-ROM), 75(1), 2020
ラトランキュリンAはF型からG型へのコンフォメーション変化を誘起しアクチン脱重合を促進する

武田修一, 藤原郁子, 小田俊郎, 成田哲博, 前田雄一郎

日本結晶学会年会講演要旨集, 2019, 2019
高分解能X線結晶構造から明らかとなったアクチン重合・ATP加水分解機構

武田修一, 成田哲博, 小田俊郎, 田中康太郎, 小池亮太郎, 太田元規, 藤原郁子, 渡邉信久, 渡邉信久, 前田雄一郎

日本結晶学会年会講演要旨集, 2017, 2017
Direct observation of branching in actin filaments triggered by Arp2/3 complex

Fujiwara, I, S Suetsugu, S Uemura, H Miki, T Takenawa, S Ishiwata

BIOPHYSICAL JOURNAL, 82(1) 385A-385A, Jan, 2002
Microscopic analysis of polymerization process of single actin filaments.

Fujiwara, I, S Takahashi, H Tadakuma, T Funatsu, S Ishiwata

BIOPHYSICAL JOURNAL, 80(1) 94A-94A, Jan, 2001

Books and Other Publications

一分子生物学

藤原郁子, 原田慶恵 (Role: Contributor)

同仁化学出版, Jun 1, 2014

Presentations

Purification and characterization of bacterial actin MreB1: a component of swimming machinery in Spiroplasma

The 61st Annual Meeting of the Biophysical Society of Japan, Nov 16, 2023
Measurement of motor protein ATPase and binding dissociation by QCM

The 61st Annual Meeting of the Biophysical Society of Japan, Nov 14, 2023
Understanding the orientation of actin subunits by conjugating with jasplakinolide or phalloidin through FRET observation

The 61st Annual Meeting of the Biophysical Society of Japan, Nov 14, 2023
QCM may detect changes in actin filaments brought by the association and dissociation of myosin

The 61st Annual Meeting of the Biophysical Society of Japan, Nov 14, 2023
Allosteric inhibitory mechanisms of actin polymerization by;cytochalasin D

The 61st Annual Meeting of the Biophysical Society of Japan, Nov 14, 2023
真菌毒サイトカラシンDはアクチン線維を切断する

三谷隆大, 武田修一, 藤原郁子, 本多元

生体運動研究合同班会議, Jan 7, 2023
Unique frequency-shifts in QCM measurement on binding biomolecules having filamentous shape

Naoki Matsumoto, Honoka Kobayashi, Taiki Nishimura, Yuki Sakurai, Kaito Kobayashi, Kaho Yokomuro, Kazuya Soda, Ikuko Fujiwara, Hajime Honda

Sep 30, 2022
Local conformational changes and the propagation along an actin filament during in vitro motility assay

Kuruto Toda, Hirotaka Itou, Ichiro Nishikata, Kenji Kamimura, Ikuko Hujiwara, Hajime Honda

Sep 29, 2022
Filament structural polymorphism of bacterial actin MreB

Daichi Takahashi, Ikuko Fujiwara, Akihiro Narita, Makoto Miyata

Sep 29, 2022
The relation between sliding velocities and frequency changes of actomyosin on the QCM

Taiki Nisimura, Naoki Matumoto, Honoka Kobayasi, Yuuki Sakurai, Kaito Kobayasi, Kaho Yokomuro, Ikuko Hujiwara, Hajime Honda

Sep 28, 2022
Inhibitory mechanism of cytochalasin D on actin by TIRF observations

Takahiro Mitani, Hikaru Empuku, Shuichi Takeda, Ikuko Fujiwara, Hajime Honda

Sep 28, 2022
Sliding mechanism of actomyosin motility assay measured from frequency change by QCM

Honoka Kobayashi, Naoki Matsumoto, Taiki Nishimura, Yuki Sakurai, Kaho Yokomuro, Kazuya Soda, Ikuko Fujiwara, Hajime Honda

Sep 28, 2022
Fluctuation difference in actin filaments bound Jasplakinolide or Phalloidin was visualized by using FRET

Ai Takahashi, Miku Nezasa, Kuruto Toda, Irfan Huzifah Ahmad, Ichiro Nishikata, Kenji Kamimura, Ikuko Fujiwara, Hajime Honda

Sep 29, 2022
水晶振動子微量天秤(QCM)を用いたアクチンとミオシンの相互作用の計測

藤原郁子, 櫻井悠貴, 松本直樹, 小林穂乃香, 本多元

生体運動班研究合同班会議, Jan 9, 2022
アクチンフィラメントの分子間FRETを使うとこんな事がわかる。

本多元, 根笹未来, 猿子良太, 西方一路, 上村健二, 藤原郁子

生体運動班研究合同班会議, Jan 8, 2022
細菌アクチンMreBのATP加水分解機構

高橋大地, 藤原郁子, 今田勝巳, 宮田真人

生体運動班研究合同班会議, Jan 8, 2022
アクチン脱重合でのサブユニット解離の観察に向けて

三谷隆大、國田樹、藤原郁子、本多元

生体運動班研究合同班会議, Jan 8, 2022
ツインフィリンC末端尾部によるアクチンキャッピングタンパク質制御機構

武田修一, 小池亮太郎, 藤原郁子, 成田哲博, 宮田真人, 太田元規, 前田雄一郎

生体運動班研究合同班会議, Jan 7, 2022
アクチンのATP加水分解反応メカニズム：ADP-Pi結合型の再発見

前田雄一郎, 兼松佑典, 武田修一, 成田哲博, 小田俊郎, 小池亮太郎, 太田元規, 岩佐充貞, 鷹野優, 森次圭, 藤原郁子, 田中康太郎, 永江峰幸, 故渡邉信久

生体運動班研究合同班会議, Jan 7, 2022
Direct observation of the polymerization and depolymerization inhibitors at the end of actin filaments

Ikuko Fujiwara, Hikaru Empuku, Naomi Courtemanche, Thomas D. Pollard, Takahiro Mitani, Moka Ito, Ichiro Nishikata, Miku Nezasa, Kenji Kamimura, Hajime Honda

Dec 3, 2021

Professional Memberships

Nov, 2020 - Present

The Molecular Biology Society of Japan
The American Society for Cell Biology
Japan Society for Cell Biology
The Biophysical Society of Japan

Research Projects

TIRF visualization of the polymerization dynamics of bacterial actin cytoskeleton, MreB and A22 driven disruption mechanism

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C), Grant-in-Aid for Scientific Research (C), Japan Society for the Promotion of Science, Apr, 2020 - Mar, 2023
Visualization of fluorescent labeled proteins required for helical-shaped bacterial swimming motility and the force propagation

Research Foundation of Opto-Science and Technology, Dec, 2019 - Dec, 2021
ゲルゾリン様タンパク質によるアクチン繊維切断機構の解明

科学研究費助成事業, 基盤研究(C), 日本学術振興会, Apr, 2017 - Mar, 2020

小田俊郎
細胞伸張制御におけるアクチン重合調節タンパク質CPの役割

科学研究費助成事業, 若手研究(B), 日本学術振興会, Apr, 2015 - Mar, 2017

藤原郁子
単一アクチンフィラメント直視による重合・脱重合ダイナミクス機構の解明

科学研究費助成事業, 特別研究員奨励費, 日本学術振興会, 2001 - 2002

藤原郁子

Academic Activities

「生物物理学を語る――研究最前線の若い女性研究者たち」

美宅成樹 (生物物理学会会長) (横浜), Dec, 2007

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Research History

Education

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Awards

Papers

Misc.

Books and Other Publications

Presentations

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