2017年9月
Selective fluorescence detection method for selenide and selenol using monochlorobimane
ANALYTICAL BIOCHEMISTRY
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- ,
- ,
- 巻
- 532
- 号
- 開始ページ
- 1
- 終了ページ
- 8
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.ab.2017.05.023
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
The low redox potential of selenide and selenol is physiologically important, as it confers efficient catalytic abilities to selenoproteins. Quantitative determination of selenol and selenide provide important clues for understanding the metabolism and physiological function of selenium. However, selective detection of selenol and selenide is extremely difficult because of their chemical similarity to thiol and sulfide. In this study, we established a highly sensitive, selective, quantitative, and simple method for detection of selenol and selenide, using a reaction with monochlorobimane (MCB), followed by ethyl acetate extraction of the product syn-(methyl,methyl)bimane. We analyzed selenide production from selenite, catalyzed by human glutathione reductase, and also determined selenide and selenol concentrations in Hepa1-6 cells using the MCB method, to demonstrate its practical applications. This study provides a new tool for selenium detection in biology. (C) 2017 Elsevier Inc. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.ab.2017.05.023
- ISSN : 0003-2697
- eISSN : 1096-0309
- PubMed ID : 28552757
- Web of Science ID : WOS:000405979000001