2012年5月
A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination
NEUROSCIENCE RESEARCH
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- 巻
- 73
- 号
- 1
- 開始ページ
- 85
- 終了ページ
- 91
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.neures.2012.02.003
- 出版者・発行元
- ELSEVIER IRELAND LTD
Reporters of Cre and/or Flp activity are important for defining the spatial and temporal extent of Cre/Flp-mediated recombination. Here, we describe R26-CAG-LF-mTFP1, a multifunctional fluorescent reporter mouse that strongly expresses mTFP1 (bright teal fluorescent protein) after Cre- and Flp-mediated recombination. To meet the need for single recombinase-mediated reporter expression, we generated derivatives of R26-CAG-LF-mTFP1. The germline excision of the Frt-flanked stop cassette in R26-CAG-LF-mTFP1 generated a Cre-dependent reporter (R26-CAG-LoxP-mTFP1). Similarly, R26-CAG-FRT-mTFP1, in which the loxP-flanked stop cassette was excised in the germline, requires only Flp to activate mTFP1 expression. (c) 2012 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.neures.2012.02.003
- ISSN : 0168-0102
- PubMed ID : 22343123
- Web of Science ID : WOS:000303899800013