論文

査読有り
2015年8月4日

Real-time imaging of bHLH transcription factors reveals their dynamic control in the multipotency and fate choice of neural stem cells

Frontiers in Cellular Neuroscience
  • Itaru Imayoshi
  • ,
  • Fumiyoshi Ishidate
  • ,
  • Ryoichiro Kageyama

9
AUGUST
開始ページ
288
終了ページ
記述言語
英語
掲載種別
DOI
10.3389/fncel.2015.00288
出版者・発行元
Frontiers Media S.A.

The basic-helix-loop-helix (bHLH) transcription factors Ascl1/Mash1, Hes1, and Olig2 regulate the fate choice of neurons, astrocytes, and oligodendrocytes, respectively
however, these factors are coexpressed in self-renewing multipotent neural stem cells (NSCs) even before cell fate determination. This fact raises the possibility that these fate determination factors are differentially expressed between self-renewing and differentiating NSCs with unique expression dynamics. Real-time imaging analysis utilizing fluorescent proteins is a powerful strategy for monitoring expression dynamics. Fusion with fluorescent reporters makes it possible to analyze the dynamic behavior of specific proteins in living cells. However, it is technically challenging to conduct long-term imaging of proteins, particularly those with low expression levels, because a high-sensitivity and low-noise imaging system is required, and very often bleaching of fluorescent proteins and cell toxicity by prolonged laser exposure are problematic. Furthermore, to analyze the functional roles of the dynamic expression of cellular proteins, it is essential to image reporter fusion proteins that are expressed at comparable levels to their endogenous expression. In this review, we introduce our recent reports about the dynamic control of bHLH transcription factors in multipotency and fate choice of NSCs, focusing on real-time imaging of fluorescent reporters fused with bHLH transcription factors. Our imaging results indicate that bHLH transcription factors are expressed in an oscillatory manner by NSCs, and that one of them becomes dominant during fate choice. We propose that the multipotent state of NSCs correlates with the oscillatory expression of several bHLH transcription factors, whereas the differentiated state correlates with the sustained expression of a single bHLH transcription factor.

リンク情報
DOI
https://doi.org/10.3389/fncel.2015.00288
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26300726
ID情報
  • DOI : 10.3389/fncel.2015.00288
  • ISSN : 1662-5102
  • PubMed ID : 26300726
  • SCOPUS ID : 84940189063

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