2011年
High Glucose Increases Metallothionein Expression in Renal Proximal Tubular Epithelial Cells
Experimental Diabetes Research
- 巻
- 2011
- 号
- 開始ページ
- 1
- 終了ページ
- 8
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1155/2011/534872
- 出版者・発行元
- Hindawi Limited
Metallothionein (MT) is an intracellular metal-binding, cysteine-rich protein, and is a potent antioxidant that protects cells and tissues from oxidative stress. Although the major isoforms MT-1 and -2 (MT-1/-2) are highly inducible in many tissues, the distribution and role of MT-1/-2 in diabetic nephropathy are poorly understood. In this study, diabetes was induced in adult male rats by streptozotocin, and renal tissues were stained with antibodies for MT-1/-2. MT-1/-2 expression was also evaluated in mProx24 cells, a mouse renal proximal tubular epithelial cell line, stimulated with high glucose medium and pretreated with the antioxidant vitamin E. MT-1/-2 expression was gradually and dramatically increased, mainly in the proximal tubular epithelial cells and to a lesser extent in the podocytes in diabetic rats, but was hardly observed in control rats. MT-1/-2 expression was also increased by high glucose stimulation in mProx24 cells. Because the induction of MT was suppressed by pretreatment with vitamin E, the expression of MT-1/-2 is induced, at least in part, by high glucose-induced oxidative stress. These observations suggest that MT-1/-2 is induced in renal proximal tubular epithelial cells as an antioxidant to protect the kidney from oxidative stress, and may offer a novel therapeutic target against diabetic nephropathy.
- リンク情報
-
- DOI
- https://doi.org/10.1155/2011/534872
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/21960990
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3179884
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000296809500001&DestApp=WOS_CPL
- URL
- http://downloads.hindawi.com/journals/jdr/2011/534872.pdf
- URL
- http://downloads.hindawi.com/journals/jdr/2011/534872.xml
- ID情報
-
- DOI : 10.1155/2011/534872
- ISSN : 1687-5214
- eISSN : 1687-5303
- PubMed ID : 21960990
- PubMed Central 記事ID : PMC3179884
- Web of Science ID : WOS:000296809500001