論文

国際誌
2020年12月1日

Oxytocin-Dependent Regulation of TRPs Expression in Trigeminal Ganglion Neurons Attenuates Orofacial Neuropathic Pain Following Infraorbital Nerve Injury in Rats.

International journal of molecular sciences
  • Masatoshi Ando
  • ,
  • Yoshinori Hayashi
  • ,
  • Suzuro Hitomi
  • ,
  • Ikuko Shibuta
  • ,
  • Akihiko Furukawa
  • ,
  • Tatsuki Oto
  • ,
  • Takanobu Inada
  • ,
  • Tomoyuki Matsui
  • ,
  • Chikashi Fukaya
  • ,
  • Noboru Noma
  • ,
  • Masakazu Okubo
  • ,
  • Yoshiyuki Yonehara
  • ,
  • Tadayoshi Kaneko
  • ,
  • Koichi Iwata
  • ,
  • Masamichi Shinoda

21
23
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/ijms21239173

We evaluated the mechanisms underlying the oxytocin (OXT)-induced analgesic effect on orofacial neuropathic pain following infraorbital nerve injury (IONI). IONI was established through tight ligation of one-third of the infraorbital nerve thickness. Subsequently, the head withdrawal threshold for mechanical stimulation (MHWT) of the whisker pad skin was measured using a von Frey filament. Trigeminal ganglion (TG) neurons innervating the whisker pad skin were identified using a retrograde labeling technique. OXT receptor-immunoreactive (IR), transient receptor potential vanilloid 1 (TRPV1)-IR, and TRPV4-IR TG neurons innervating the whisker pad skin were examined on post-IONI day 5. The MHWT remarkably decreased from post-IONI day 1 onward. OXT application to the nerve-injured site attenuated the decrease in MHWT from day 5 onward. TRPV1 or TRPV4 antagonism significantly suppressed the decrement of MHWT following IONI. OXT receptors were expressed in the uninjured and Fluoro-Gold (FG)-labeled TG neurons. Furthermore, there was an increase in the number of FG-labeled TRPV1-IR and TRPV4-IR TG neurons, which was inhibited by administering OXT. This inhibition was suppressed by co-administration with an OXT receptor antagonist. These findings suggest that OXT application inhibits the increase in TRPV1-IR and TRPV4-IR TG neurons innervating the whisker pad skin, which attenuates post-IONI orofacial mechanical allodynia.

リンク情報
DOI
https://doi.org/10.3390/ijms21239173
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33271955
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7731199
ID情報
  • DOI : 10.3390/ijms21239173
  • PubMed ID : 33271955
  • PubMed Central 記事ID : PMC7731199

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