論文

査読有り
2007年9月

Formation of a FGF-2 and calcium phosphate composite layer on a hydroxyapatite ceramic for promoting bone formation

BIOMEDICAL MATERIALS
  • Yu Sogo
  • ,
  • Atsuo Ito
  • ,
  • Masahiro Onoguchi
  • ,
  • Ayako Oyane
  • ,
  • Hideo Tsurushima
  • ,
  • Noboru Ichinose

2
3
開始ページ
S175
終了ページ
S180
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1088/1748-6041/2/3/S16
出版者・発行元
IOP PUBLISHING LTD

Fibroblast growth factor-2 (FGF-2) was immobilized on a hydroxyapatite (HAP) ceramic in supersaturated calcium phosphate solution prepared using solutions corresponding to clinically approved infusion fluids. To avoid the risk of FGF-2 denaturation, FGF-2 immobilization was carried out at 25 degrees C. FGF-2 was successfully immobilized on HAP ceramic surfaces by deposition with calcium phosphate to form a FGF-2 and calcium phosphate composite layer. A maximum of 2.72 +/- 0.01 mu g cm(-2) of FGF-2 was immobilized in the composite layer formed on the HAP ceramic under the optimum condition. A FGF-2-immobilized HAP ceramic is likely to have the ability to release a sufficient amount of FGF-2 to promote bone formation. FGF-2 released from a FGF-2-immobilized HAP ceramic maintained its biological activity, since the proliferation of fibroblastic NIH3T3 was promoted. Therefore, the FGF-2-immobilized HAP ceramic is expected to be a useful material for promoting new bone formation.

Web of Science ® 被引用回数 : 29

リンク情報
DOI
https://doi.org/10.1088/1748-6041/2/3/S16
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000249597800017&DestApp=WOS_CPL
ID情報
  • DOI : 10.1088/1748-6041/2/3/S16
  • ISSN : 1748-6041
  • Web of Science ID : WOS:000249597800017

エクスポート
BibTeX RIS