論文

査読有り
2009年1月29日

Identification of retinal ganglion cells and their projections involved in central transmission of information about upward and downward image motion.

PloS one
  • Hiroshi Ishikane

4
1
開始ページ
e4320
終了ページ
e4320
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0004320
出版者・発行元
Public Library of Science (PLoS)

The direction of image motion is coded by direction-selective (DS) ganglion cells in the retina. Particularly, the ON DS ganglion cells project their axons specifically to terminal nuclei of the accessory optic system (AOS) responsible for optokinetic reflex (OKR). We recently generated a knock-in mouse in which SPIG1 (SPARC-related protein containing immunoglobulin domains 1)-expressing cells are visualized with GFP, and found that retinal ganglion cells projecting to the medial terminal nucleus (MTN), the principal nucleus of the AOS, are comprised of SPIG1+ and SPIG1(-) ganglion cells distributed in distinct mosaic patterns in the retina. Here we examined light responses of these two subtypes of MTN-projecting cells by targeted electrophysiological recordings. SPIG1+ and SPIG1(-) ganglion cells respond preferentially to upward motion and downward motion, respectively, in the visual field. The direction selectivity of SPIG1+ ganglion cells develops normally in dark-reared mice. The MTN neurons are activated by optokinetic stimuli only of the vertical motion as shown by Fos expression analysis. Combination of genetic labeling and conventional retrograde labeling revealed that axons of SPIG1+ and SPIG1(-) ganglion cells project to the MTN via different pathways. The axon terminals of the two subtypes are organized into discrete clusters in the MTN. These results suggest that information about upward and downward image motion transmitted by distinct ON DS cells is separately processed in the MTN, if not independently. Our findings provide insights into the neural mechanisms of OKR, how information about the direction of image motion is deciphered by the AOS.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0004320
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19177171
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2629575
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000265483100007&DestApp=WOS_CPL
URL
http://dx.plos.org/10.1371/journal.pone.0004320
ID情報
  • DOI : 10.1371/journal.pone.0004320
  • ISSN : 1932-6203
  • eISSN : 1932-6203
  • ORCIDのPut Code : 99797827
  • PubMed ID : 19177171
  • PubMed Central 記事ID : PMC2629575
  • Web of Science ID : WOS:000265483100007

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