論文

査読有り
2013年9月

N-Cadherin is a prospective cell surface marker of human mesenchymal stem cells that have high ability for cardiomyocyte differentiation

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Hisako Ishimine
  • Norio Yamakawa
  • Mari Sasao
  • Mika Tadokoro
  • Daisuke Kami
  • Shinji Komazaki
  • Makoto Tokuhara
  • Hitomi Takada
  • Yoshimasa Ito
  • Shinichiro Kuno
  • Kotaro Yoshimura
  • Akihiro Umezawa
  • Hajime Ohgushi
  • Makoto Asashima
  • Akira Kurisaki
  • 全て表示

438
4
開始ページ
753
終了ページ
759
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2013.07.081
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

Mesenchymal stem cells (MSCs) are among the most promising sources of stem cells for regenerative medicine. However, the range of their differentiation ability is very limited. In this study, we explored prospective cell surface markers of human MSCs that readily differentiate into cardiomyocytes. When the cardiomyogenic differentiation potential and the expression of cell surface markers involved in heart development were analyzed using various immortalized human MSC lines, the MSCs with high expression of N-cadherin showed a higher probability of differentiation into beating cardiomyocytes. The differentiated cardiomyocytes expressed terminally differentiated cardiomyocyte-specific markers such as alpha-actinin, cardiac troponin T, and connexin-43. A similar correlation was observed with primary human MSCs derived from bone marrow and adipose tissue. Moreover, N-cadherin-positive MSCs isolated with N-cadherin antibody-conjugated magnetic beads showed an apparently higher ability to differentiate into cardiomyocytes than the N-cadherin-negative population. Quantitative polymerase chain reaction analyses demonstrated that the N-cadherin-positive population expressed significantly elevated levels of cardiomyogenic progenitor-specific transcription factors, including Nkx2.5, Hand1, and GATA4 mRNAs. Our results suggest that N-cadherin is a novel prospective cell surface marker of human MSCs that show a better ability for cardiomyocyte differentiation. (C) 2013 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2013.07.081
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23899519
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000324454800030&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.bbrc.2013.07.081
  • ISSN : 0006-291X
  • PubMed ID : 23899519
  • Web of Science ID : WOS:000324454800030

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