論文

査読有り
2016年11月

A reliable aptamer array prepared by repeating inkjet-spotting toward on-site measurement

BIOSENSORS & BIOELECTRONICS
  • Suzuyo Inoue
  • ,
  • Michiko Seyama
  • ,
  • Toru Miura
  • ,
  • Tsutomu Horiuchi
  • ,
  • Yuzuru Iwasaki
  • ,
  • Jun-ichi Takahashi
  • ,
  • Katsuyoshi Hayashi
  • ,
  • Emi Tamechika

85
開始ページ
943
終了ページ
949
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bios.2016.05.089
出版者・発行元
ELSEVIER ADVANCED TECHNOLOGY

A preparation protocol is proposed for a reliable aptamer array utilizing an ink-jet spotter. We accumulated streptavidin and biotinylated-aptamer in this order on a biotinylated-polyethylene glycol-coated gold substrate to prepare an aptamer array. The aptamer array was prepared with an alternate spotting structure where each aptamer spot was placed between reference spots formed with blocking solution thus suppressing contamination from neighboring spots during the blocking and washing processes. Four aptamer spots were prepared in a small area of 1 x 4.8 mm(2) with five reference spots made of blocking solution. We evaluated the thrombin binding ability of the spotted aptamer array using a multi analysis surface plasmon resonance sensor. We prepared a disposable capillary-driven flow chip designed for on-site measurement (Miura et al., 2010) with our aptamer array and detected thrombin from phosphate-buffered saline at concentrations of 50 ng mL(-1) and 1 mu g mL(-1) (equivalent to 1.35 and 27 nM, respectively). A correlation was observed between the refractive index shift and thrombin concentration. This implies that our array preparation protocol meets the requirement for the preparation of a one-time-use chip for on-site measurement. (C) 2016 Elsevier B.V. All rights reserved.

Web of Science ® 被引用回数 : 5

リンク情報
DOI
https://doi.org/10.1016/j.bios.2016.05.089
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000382410100127&DestApp=WOS_CPL
URL
http://orcid.org/0000-0001-6462-1453

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