論文

査読有り
2015年9月

Polymer brush biointerfaces for highly sensitive biosensors that preserve the structure and function of immobilized proteins

SENSORS AND ACTUATORS B-CHEMICAL
  • Kenji Takasu
  • ,
  • Keiichiro Kushiro
  • ,
  • Katsuyoshi Hayashi
  • ,
  • Yuzuru Iwasaki
  • ,
  • Suzuyo Inoue
  • ,
  • Emi Tamechika
  • ,
  • Madoka Takai

216
0
開始ページ
428
終了ページ
433
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.snb.2015.04.056
出版者・発行元
ELSEVIER SCIENCE SA

In order to develop highly sensitive and high-throughput protein assay systems for proteomics and biosensor systems, we elucidated the relationship between protein structure and activity on various polymer brush surfaces that immobilize proteins through different mechanisms. Various polymer brush surfaces (cationic, hydrophobic, covalent-bonding, and block-type with the cationic and zwitterionic) were synthesized via surface-initiated atom transfer radical polymerization (SI-ATRP), and both the secondary structure and activity of proteins immobilized on each surface were investigated by circular dichroism and equilibrium dissociation constant (K-d) analysis, respectively. There was a strong correlation between the stability of the secondary structures and the activity of the proteins on the polymer brush surfaces. Interestingly, proteins immobilized on the block-type polymer surface (PMPC-block-PAEMA (PMbA)) showed less structural change and higher activity than the other polymer brush surfaces. These results suggest that the prevention of the adsorption-induced structural changes has a great role in improving the activity of immobilized proteins, and underscore the potentials of zwitterionic polymers for creating ideal biointerfaces derived from the functional, surface-immobilized proteins. Such biointerfaces can be useful for improving the specificity and sensitivity of protein microarrays and biosensor systems. (C) 2015 Elsevier B.V. All rights reserved.

Web of Science ® 被引用回数 : 18

リンク情報
DOI
https://doi.org/10.1016/j.snb.2015.04.056
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000355131800057&DestApp=WOS_CPL
URL
http://orcid.org/0000-0001-6462-1453

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