Jun, 1995
IDIOTYPIC DIVERSITY AND VARIABLE REGION GENE USAGE BY MOUSE ANTI-HLA-DQ3 MAB
IMMUNOGENETICS
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- Volume
- 42
- Number
- 2
- First page
- 90
- Last page
- 100
- Language
- English
- Publishing type
- Research paper (scientific journal)
- Publisher
- SPRINGER VERLAG
The anti-HLA-DQ3 monoclonal antibodies (mAb) KS13, SO1, SO2, SO3, SO4, and SO5 recognize spatially close but distinct antigenic determinants, since they crossinhibit each other in their binding to HLA-DQ3 antigens, but do not share idiotopes recognized in their antigen combining site by syngeneic and anti-id antisera and mAb. Furthermore, mAb SO1, SO3, SO4, and SO5 react also with HLA-DQ allospecificities other than HLA-DQ3. Sequence analysis of the heavy (V-H) and light (V-L) chain variable region of the six mAb revealed preferential usage of V-H 36-60 and V-K 12/13 gene families. However, the individual V-H and V-L germline gene usage by the six mAb is diverse and the utilization of D, J(H), and J(L) gene segments is heterogeneous. The diverse usage of V-H and V-L. gene segments and heterogeneous amino acid sequences of V-H and V-L CDR, together with the heterogeneous idiotypic profile, may reflect the complexity of the determinants recognized by the six mAb on HLA-DQ3 antigens. The results we have presented provide for the first time information about the structural basis of the diversity of antibodies recognizing human histocompatibility antigens.
- Link information
- ID information
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- ISSN : 0093-7711
- Web of Science ID : WOS:A1995RF71000003