The Mott insulator is found in the two-channel Kondo lattice by using the dynamical mean-field theory combined with continuous-time quantum Monte Carlo method. At quarter filling of two conduction bands, the paramagnetic state shows metal-insulator crossover with increasing the coupling constant at low temperatures. The insulating state is explained by the strong coupling limit, where the system is effectively interpreted as the half-filled Hubbard model. It is shown that the present Mott insulating state is well described by the Hubbard I approximation. © Published under licence by IOP Publishing Ltd.