2013年8月
Expression, purification, crystallization and preliminary crystallographic study of FtsA from methicillin-resistant Staphylococcus aureus.
Acta crystallographica. Section F, Structural biology and crystallization communications
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- 巻
- 69
- 号
- Pt 8
- 開始ページ
- 895
- 終了ページ
- 898
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1107/S1744309113017727
- 出版者・発行元
- International Union of Crystallography ({IUCr})
FtsA from methicillin-resistant Staphylococcus aureus (MRSA) was cloned, overexpressed and purified. The protein was crystallized using the sitting-drop vapour-diffusion technique. A cocrystal with β-γ-imidoadenosine 5'-phosphate (AMPPNP; a nonhydrolysable ATP analogue) was grown using PEG 3350 as a precipitant at 293 K. X-ray diffraction data were collected to a resolution of 2.3 Å at 100 K. The crystal belonged to the monoclinic space group P2₁, with unit-cell parameters a = 75.31, b = 102.78, c = 105.90 Å, β = 96.54°. The calculated Matthews coefficient suggested that the asymmetric unit contained three or four monomers.
- リンク情報
- ID情報
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- DOI : 10.1107/S1744309113017727
- ISSN : 1744-3091
- ORCIDのPut Code : 47596989
- PubMed ID : 23908037
- PubMed Central 記事ID : PMC3729168