論文

査読有り
2016年12月

Possible association of diazotrophs with marine zooplankton in the Pacific Ocean

MICROBIOLOGYOPEN
  • Kazi Md. Azimuddin
  • ,
  • Junya Hirai
  • ,
  • Shotaro Suzuki
  • ,
  • Md. Nurul Haider
  • ,
  • Aiko Tachibana
  • ,
  • Keigo Watanabe
  • ,
  • Minoru Kitamura
  • ,
  • Fuminori Hashihama
  • ,
  • Kazutaka Takahashi
  • ,
  • Koji Hamasaki

5
6
開始ページ
1016
終了ページ
1026
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/mbo3.385
出版者・発行元
WILEY-BLACKWELL

Dinitrogen fixation, the biological reduction in N-2 gas to ammonia contributes to the supply of new nitrogen in the surface ocean. To understand the diversity and abundance of potentially diazotrophic (N-2 fixing) microorganisms associated with marine zooplankton, especially copepods, the nifH gene was studied using zooplankton samples collected in the Pacific Ocean. In total, 257 nifH sequences were recovered from 23 nifH-positive DNA extracts out of 90 copepod samples.The nifH genes derived from cyanobacteria related to Trichodesmium, - and -subdivisions of proteobacteria, and anaerobic euryarchaeota related to Methanosaeta concilii were detected. Our results indicated that Pleuromamma, Pontella, and Euchaeta were the major copepod genera hosting dinitrogen fixers, though we found no species-specific association between copepods and dinitrogen fixers. Also, the digital PCR provided novel data on the number of copies of the nifH gene in individual copepods, which we report the range from 30 to 1666 copies per copepod. This study is the first systematic study of zooplankton-associated diazotrophs, covering a large area of the open ocean, which provide a clue to further study of a possible new hotspot of N-2 fixation.

Web of Science ® 被引用回数 : 3

リンク情報
DOI
https://doi.org/10.1002/mbo3.385
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000390567300009&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/mbo3.385
  • ISSN : 2045-8827
  • Web of Science ID : WOS:000390567300009

エクスポート
BibTeX RIS