- OXFORD UNIV PRESS
We evaluated the internal transcribed spacer 2 and large subunit ribosomal DNA (ITS2-28S) region as a molecular marker for identifying calanoid copepods in the subtropical western North Pacific. The ITS2-28S sequence was successfully amplified in 232/244 individuals, a much higher rate of success than for cytochrome oxidase I amplification (77/244 individuals). A total of 194 sequences of ITS2-28S were obtained using a single primer pair. A high degree of genetic variability in ITS2-28S was observed in Lucicutia flavicornis (n 13), Nannocalanus minor (n 9), Pleuromamma abdominalis (n 9) and Spinocalanus spinosus (n 2), each showing several sequence types based on phylogenetic analysis of the ITS2-28S. A difference in the body size that corresponded with two sequence types of P. abdominalis was observed. The other species showed consistently lower genetic variability within species (00.001) than between species (0.005) using ITS2-28S of 810968 bp aligned in each family. Although further morphological and genetic analysis of a larger sample size are necessary, our results suggest that ITS2-28S is a nuclear marker that does not need to be cloned and is easily amplified and sufficiently variable as a possible marker for DNA barcoding or to identify genetic groups of calanoid copepods.
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- DOI : 10.1093/plankt/fbt016
- ISSN : 0142-7873
- Web of Science ID : WOS:000318573100014