論文

査読有り 筆頭著者 責任著者
2013年5月

Evaluation of ITS2-28S as a molecular marker for identification of calanoid copepods in the subtropical western North Pacific

JOURNAL OF PLANKTON RESEARCH
  • Junya Hirai
  • ,
  • Shinji Shimode
  • ,
  • Atsushi Tsuda

35
3
開始ページ
644
終了ページ
656
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/plankt/fbt016
出版者・発行元
OXFORD UNIV PRESS

We evaluated the internal transcribed spacer 2 and large subunit ribosomal DNA (ITS2-28S) region as a molecular marker for identifying calanoid copepods in the subtropical western North Pacific. The ITS2-28S sequence was successfully amplified in 232/244 individuals, a much higher rate of success than for cytochrome oxidase I amplification (77/244 individuals). A total of 194 sequences of ITS2-28S were obtained using a single primer pair. A high degree of genetic variability in ITS2-28S was observed in Lucicutia flavicornis (n 13), Nannocalanus minor (n 9), Pleuromamma abdominalis (n 9) and Spinocalanus spinosus (n 2), each showing several sequence types based on phylogenetic analysis of the ITS2-28S. A difference in the body size that corresponded with two sequence types of P. abdominalis was observed. The other species showed consistently lower genetic variability within species (00.001) than between species (0.005) using ITS2-28S of 810968 bp aligned in each family. Although further morphological and genetic analysis of a larger sample size are necessary, our results suggest that ITS2-28S is a nuclear marker that does not need to be cloned and is easily amplified and sufficiently variable as a possible marker for DNA barcoding or to identify genetic groups of calanoid copepods.

Web of Science ® 被引用回数 : 29

リンク情報
DOI
https://doi.org/10.1093/plankt/fbt016
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000318573100014&DestApp=WOS_CPL
ID情報
  • DOI : 10.1093/plankt/fbt016
  • ISSN : 0142-7873
  • Web of Science ID : WOS:000318573100014

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