論文

査読有り 筆頭著者 責任著者
2017年12月11日

Cryptic zooplankton diversity revealed by a metagenetic approach to monitoring metazoan communities in the coastal waters of the Okhotsk Sea, northeastern Hokkaido

Frontiers in Marine Science
  • Junya Hirai
  • ,
  • Seiji Katakura
  • ,
  • Hiromi Kasai
  • ,
  • Satoshi Nagai

4
379
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3389/fmars.2017.00379
出版者・発行元
Frontiers Media S. A

Monitoring zooplankton communities is important to understand dynamics in marine ecosystems. However, it is difficult to identify cryptic species and immature stages of zooplankton using morphological classification, which is time-consuming and requires high skill levels. Here, we conducted a metagenetic analysis of the 18S region in 101 zooplankton samples collected weekly throughout 2014 and 2015 at the Okhotsk Tower in Mombetsu, Hokkaido, Japan, and compared the results of this analysis with those provided by morphological analysis. The metagenetic analysis detected 561 molecular taxonomic units (MOTUs), whereas the morphological analysis detected 201 taxonomic groups. Zooplankton communities were dominated by copepods throughout the sampling period
however, non-copepod taxa, which comprised high proportions of both MOTUs (mean 51.1%) and sequence reads (mean 19.1%), were also important. Cryptic diversity detected by the metagenetic analysis was primarily driven by Copepoda and by the larvae of benthic taxa such as Bivalvia, Gastropoda, and Polychaeta. Community structure and diversity varied between periods of warm and cold water, indicating strong correlations with water temperature and thus seasonality. Furthermore, metagenetic analysis revealed detailed seasonal changes in dominant taxa, including larval stages of metazoans with high taxonomic resolutions
these included commercially important organisms such as Japanese scallops. The metagenetic analysis revealed that changes in both water mass and bentho-pelagic interactions sustain ecosystems rich in zooplankton diversity in this area. Metagenetic analysis provides novel insight into zooplankton diversity, and generates massive sequence data that may be used in future research
thus, it is considered an effective tool for monitoring zooplankton communities.

リンク情報
DOI
https://doi.org/10.3389/fmars.2017.00379
URL
https://www.frontiersin.org/articles/10.3389/fmars.2017.00379/full
ID情報
  • DOI : 10.3389/fmars.2017.00379
  • ISSN : 2296-7745
  • SCOPUS ID : 85037611081

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