Feb, 1999
Formation of gamma-glutamylpropargylglycylglycine from propargylglycine in human blood and erythrocytes
ACTA MEDICA OKAYAMA
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- Volume
- 53
- Number
- 1
- First page
- 19
- Last page
- 25
- Language
- English
- Publishing type
- Research paper (scientific journal)
- Publisher
- OKAYAMA UNIV MED SCHOOL
gamma-Glutamylpropargylglycylglycine (gamma-Glu-PPG-Gly) was isolated as a metabolite of propargyl-glycine (2-amino-4-pentynoic acid, a natural and synthetic inhibitor of cystathionine gamma-lyase) from human blood incubated with D,L-propargylglycine in the presence of L-glutamate and glycine, and identified by fast-atom-bombardment mass spectrometry, indicating that human blood can metabolize propargylglycine to gamma-Glu-PPG-Gly. When whole blood was incubated with 2mM D,L-propargylglycine in the presence of 10 mM L-glutamate and 10 mM glycine at 37 degrees C for 16 h, 0.094 +/- 0.013 mu mol of gamma-Glu-PPG-Gly was formed per mi of whole blood. When erythrocytes were incubated under the same conditions for 16 h, 0.323 +/- 0.060 mu mol of gamma-Glu-PPG-Gly was formed per mi of erythrocytes, suggesting a large contribution of erythrocytes to gamma-Glu-PPG-Gly formation in whole blood. The apparent K-m value of gamma-Glu-PPG-Gly formation in human erythrocytes for D,L-propargylglycine was 0.32 mM. The observed rate of gamma-Glu-PPG-Gly formation and the K-m value for D,L-propargylglycine suggest that metabolism of propargylglycine to gamma-Glu-PPG-Gly can play a definite biological role in human subjects who are loaded with propargylglycine.
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- ID information
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- ISSN : 0386-300X
- Web of Science ID : WOS:000078897700004